This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

2rm8

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Revision as of 17:57, 8 February 2016

The solution structure of phototactic transducer protein HtrII linker region from Natronomonas pharaonis

Structural highlights

2rm8 is a 1 chain structure with sequence from Atcc 35678. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	htrII (ATCC 35678)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum

Function

[HTR2_NATPH] Transduces signals from the phototaxis receptor sensory rhodopsin II (SR-II) to the flagellar motor. Responds to light changes through the variation of the level of methylation. Also acts as a chemotransducer.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Halobacterial pharaonis phoborhodopsin [ppR, also called Natronomonas pharaonis sensory rhodopsin II (NpSRII)] is a phototaxis protein which transmits a light signal to the cytoplasm through its transducer protein (pHtrII). pHtrII, a two-transmembrane protein that interacts with ppR, belongs to the group of methyl-accepting chemotaxis proteins (MCPs). Several mutation studies have indicated that the linker region connecting the transmembrane and methylation regions is necessary for signal transduction. However, the three-dimensional (3D) structure of an MCP linker region has yet to be reported, and hence, details concerning the signal transduction mechanism remain unknown. Here the structure of the pHtrII linker region was investigated biochemically and biophysically. Following limited proteolysis, only one trypsin resistant fragment in the pHtrII linker region was identified. This fragment forms a homodimer with a Kd value of 115 muM. The 3D structure of this fragment was determined by solution NMR, and only one alpha-helix was found between two HAMP domains of the linker region. This alpha-helix was significantly stabilized within transmembrane protein pHtrII as revealed by CW-EPR. The presence of Af1503 HAMP domain-like structures in the linker region was supported by CD, NMR, and ELDOR data. The alpha-helix determined here presumably works as a mechanical joint between two HAMP domains in the linker region to transfer the photoactivated conformational change downstream.

Structural Analysis of the Phototactic Transducer Protein HtrII Linker Region from Natronomonas pharaonis(,).,Hayashi K, Sudo Y, Jee J, Mishima M, Hara H, Kamo N, Kojima C Biochemistry. 2007 Dec 18;46(50):14380-14390. Epub 2007 Nov 15. PMID:18001143^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Hayashi K, Sudo Y, Jee J, Mishima M, Hara H, Kamo N, Kojima C. Structural Analysis of the Phototactic Transducer Protein HtrII Linker Region from Natronomonas pharaonis(,). Biochemistry. 2007 Dec 18;46(50):14380-14390. Epub 2007 Nov 15. PMID:18001143 doi:10.1021/bi701837n

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2rm8"

@@ Line 2: / Line 2: @@
 <StructureSection load='2rm8' size='340' side='right' caption='[[2rm8]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2rm8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Natronomonas_pharaonis Natronomonas pharaonis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RM8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2RM8 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2rm8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_35678 Atcc 35678]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RM8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2RM8 FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">htrII ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2257 Natronomonas pharaonis])</td></tr>
+</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">htrII ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2257 ATCC 35678])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rm8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rm8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2rm8 RCSB], [http://www.ebi.ac.uk/pdbsum/2rm8 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rm8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rm8 OCA], [http://pdbe.org/2rm8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2rm8 RCSB], [http://www.ebi.ac.uk/pdbsum/2rm8 PDBsum]</span></td></tr>
 </table>
 == Function ==
@@ Line 16: / Line 16: @@
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
-</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2rm8 ConSurf].
 <div style="clear:both"></div>
 <div style="background-color:#fffaf0;">
@@ Line 26: / Line 26: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 2rm8" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Natronomonas pharaonis]]
+[[Category: Atcc 35678]]
 [[Category: Hara, H]]
 [[Category: Hayashi, K]]

2rm8

From Proteopedia

Revision as of 17:57, 8 February 2016

The solution structure of phototactic transducer protein HtrII linker region from Natronomonas pharaonis

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox