This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

3zbd

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Revision as of 09:47, 5 August 2016

Structure of TGEV nsp1

Structural highlights

3zbd is a 2 chain structure with sequence from Cvppu. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[R1A_CVPPU] The papain-like proteinase 1 (PLP1) and papain-like proteinase 2 (PLP2) are responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PLP2 possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. PLP2 also antagonizes innate immune induction of type I interferon by blocking the nuclear translocation of host IRF-3 (By similarity). The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SAGC]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter (By similarity). Nsp9 is a ssRNA-binding protein (By similarity).

Publication Abstract from PubMed

Coronavirus nsp1 have been shown to induce suppression of host mRNA expression and to interfere with host immune response. However, the mechanism is currently unknown. The only available structural information on coronavirus nsp1 is the NMR structure of the N-terminal domain of nsp1 from severe acute respiratory syndrom coronavirus (SARS-CoV) from the genus betacoronavirus. Here we present the first nsp1 structure from an alphacoronavirus, TGEV nsp1. It displays a six-stranded beta-barrel fold with a long alpha helix on the rim of the barrel, a fold shared with SARS-CoV nsp1(13-128). Contrary to previous speculation, the TGEV nsp1 structure suggests that coronavirus nsp1s have a common origin, despite the lack of sequence homology. However, comparisons of surface electrostatics, shape and amino acid conservation between the alpha- and betacoronaviruses lead us to speculate that the mechanism for nsp1 induced suppression of host mRNA expression might be different in these two genera.

Structure of alphacoronavirus TGEV nsp1 has implications for coronavirus nsp1 function and evolution.,Jansson AM J Virol. 2012 Dec 26. PMID:23269811^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Jansson AM. Structure of alphacoronavirus TGEV nsp1 has implications for coronavirus nsp1 function and evolution. J Virol. 2012 Dec 26. PMID:23269811 doi:http://dx.doi.org/10.1128/JVI.03163-12

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3zbd"

@@ Line 1: / Line 1: @@
 ==Structure of TGEV nsp1==
 <StructureSection load='3zbd' size='340' side='right' caption='[[3zbd]], [[Resolution|resolution]] 1.49&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3zbd]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Porcine_transmissible_gastroenteritis_coronavirus_strain_purdue Porcine transmissible gastroenteritis coronavirus strain purdue]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZBD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZBD FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3zbd]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cvppu Cvppu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZBD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZBD FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zbd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zbd OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zbd RCSB], [http://www.ebi.ac.uk/pdbsum/3zbd PDBsum]</span></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zbd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zbd OCA], [http://pdbe.org/3zbd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3zbd RCSB], [http://www.ebi.ac.uk/pdbsum/3zbd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3zbd ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 15: / Line 16: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 3zbd" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Porcine transmissible gastroenteritis coronavirus strain purdue]]
+[[Category: Cvppu]]
 [[Category: Jansson, A M]]
 [[Category: Alphacoronavirus]]

3zbd

From Proteopedia

Revision as of 09:47, 5 August 2016

Structure of TGEV nsp1

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox