2au1
From Proteopedia
| Line 7: | Line 7: | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2au1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2au1 OCA], [http://www.ebi.ac.uk/pdbsum/2au1 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2au1 RCSB]</span> | ||
}} | }} | ||
| Line 16: | Line 19: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2AU1 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ | + | 2AU1 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptococcus_pyogenes_mgas5005 Streptococcus pyogenes mgas5005]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AU1 OCA]. |
==Reference== | ==Reference== | ||
Crystal structure of group A streptococcus Mac-1: insight into dimer-mediated specificity for recognition of human IgG., Agniswamy J, Nagiec MJ, Liu M, Schuck P, Musser JM, Sun PD, Structure. 2006 Feb;14(2):225-35. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16472742 16472742] | Crystal structure of group A streptococcus Mac-1: insight into dimer-mediated specificity for recognition of human IgG., Agniswamy J, Nagiec MJ, Liu M, Schuck P, Musser JM, Sun PD, Structure. 2006 Feb;14(2):225-35. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16472742 16472742] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Streptococcus pyogenes | + | [[Category: Streptococcus pyogenes mgas5005]] |
[[Category: Agniswamy, J.]] | [[Category: Agniswamy, J.]] | ||
[[Category: Liu, M.]] | [[Category: Liu, M.]] | ||
| Line 28: | Line 31: | ||
[[Category: Schuck, P.]] | [[Category: Schuck, P.]] | ||
[[Category: Sun, P D.]] | [[Category: Sun, P D.]] | ||
| - | [[Category: BME]] | ||
[[Category: mac-1]] | [[Category: mac-1]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:57:16 2008'' |
Revision as of 22:57, 30 March 2008
| |||||||
| , resolution 2.4Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of group A Streptococcus MAC-1 orthorhombic form
Overview
Group A Streptococcus secretes cysteine proteases named Mac-1 and Mac-2 that mediate host immune evasion by targeting both IgG and Fc receptors. Here, we report the crystal structures of Mac-1 and its catalytically inactive C94A mutant in two different crystal forms. Despite the lack of sequence homology, Mac-1 adopts the canonical papain fold. Alanine mutations at the active site confirmed the critical residues involved in a papain-like catalytic mechanism. Mac-1 forms a symmetric dimer in both crystal forms and displays the unique dimer interface among papain superfamily members. Mutations at the dimer interface resulted in a significant reduction in IgG binding and catalysis, suggesting that the dimer contributes to both IgG specificity and enzyme cooperativity. A tunnel observed at the dimer interface constitutes a target for designing potential Mac-1-specific antimicrobial agents. The structures also offer insight into the functional difference between Mac-1 and Mac-2.
About this Structure
2AU1 is a Single protein structure of sequence from Streptococcus pyogenes mgas5005. Full crystallographic information is available from OCA.
Reference
Crystal structure of group A streptococcus Mac-1: insight into dimer-mediated specificity for recognition of human IgG., Agniswamy J, Nagiec MJ, Liu M, Schuck P, Musser JM, Sun PD, Structure. 2006 Feb;14(2):225-35. PMID:16472742
Page seeded by OCA on Mon Mar 31 01:57:16 2008
