2axc
From Proteopedia
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|PDB= 2axc |SIZE=350|CAPTION= <scene name='initialview01'>2axc</scene>, resolution 1.70Å | |PDB= 2axc |SIZE=350|CAPTION= <scene name='initialview01'>2axc</scene>, resolution 1.70Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= colE7 (AMINO ACIDS 60-316) ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | |GENE= colE7 (AMINO ACIDS 60-316) ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1m08|1M08]], [[7cei|7CEI]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2axc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2axc OCA], [http://www.ebi.ac.uk/pdbsum/2axc PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2axc RCSB]</span> | ||
}} | }} | ||
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[[Category: Yang, W Z.]] | [[Category: Yang, W Z.]] | ||
[[Category: Yuan, H S.]] | [[Category: Yuan, H S.]] | ||
| - | [[Category: GOL]] | ||
| - | [[Category: SO4]] | ||
[[Category: colicin]] | [[Category: colicin]] | ||
[[Category: translocation domain]] | [[Category: translocation domain]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:58:33 2008'' |
Revision as of 22:58, 30 March 2008
| |||||||
| , resolution 1.70Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , | ||||||
| Gene: | colE7 (AMINO ACIDS 60-316) (Escherichia coli) | ||||||
| Related: | 1M08, 7CEI
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of ColE7 translocation domain
Overview
ColE7 is a nuclease-type colicin released from Escherichia coli to kill sensitive bacterial cells by degrading the nucleic acid molecules in their cytoplasm. ColE7 is classified as one of the group A colicins, since the N-terminal translocation domain (T-domain) of the nuclease-type colicins interact with specific membrane-bound or periplasmic Tol proteins during protein import. Here, we show that if the N-terminal tail of ColE7 is deleted, ColE7 (residues 63-576) loses its bactericidal activity against E.coli. Moreover, TolB protein interacts directly with the T-domain of ColE7 (residues 1-316), but not with the N-terminal deleted T-domain (residues 60-316), as detected by co-immunoprecipitation experiments, confirming that the N-terminal tail is required for ColE7 interactions with TolB. The crystal structure of the N-terminal tail deleted ColE7 T-domain was determined by the multi-wavelength anomalous dispersion method at a resolution of 1.7 angstroms. The structure of the ColE7 T-domain superimposes well with the T-domain of ColE3 and TR-domain of ColB, a group A Tol-dependent colicin and a group B TonB-dependent colicin, respectively. The structural resemblance of group A and B colicins implies that the two groups of colicins may share a mechanistic connection during cellular import.
About this Structure
2AXC is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
High-resolution crystal structure of a truncated ColE7 translocation domain: implications for colicin transport across membranes., Cheng YS, Shi Z, Doudeva LG, Yang WZ, Chak KF, Yuan HS, J Mol Biol. 2006 Feb 10;356(1):22-31. Epub 2005 Dec 5. PMID:16360169
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