2b2d
From Proteopedia
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|PDB= 2b2d |SIZE=350|CAPTION= <scene name='initialview01'>2b2d</scene>, resolution 2.9Å | |PDB= 2b2d |SIZE=350|CAPTION= <scene name='initialview01'>2b2d</scene>, resolution 2.9Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= | + | |LIGAND= <scene name='pdbligand=A:ADENOSINE-5'-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5'-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5'-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=U:URIDINE-5'-MONOPHOSPHATE'>U</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[2bny|2BNY]], [[1aq3|1AQ3]], [[1aq4|1AQ4]], [[1bms|1BMS]], [[1dzs|1DZS]], [[1e6t|1E6T]], [[2b2e|2B2E]], [[2b2g|2B2G]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2b2d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b2d OCA], [http://www.ebi.ac.uk/pdbsum/2b2d PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2b2d RCSB]</span> | ||
}} | }} | ||
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[[Category: virus/viral]] | [[Category: virus/viral]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:00:32 2008'' |
Revision as of 23:00, 30 March 2008
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| , resolution 2.9Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , | ||||||
| Related: | 2BNY, 1AQ3, 1AQ4, 1BMS, 1DZS, 1E6T, 2B2E, 2B2G
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
RNA stemloop operator from bacteriophage QBETA complexed with an N87S,E89K mutant MS2 capsid
Overview
Sequence-specific interactions between RNA stem-loops and coat protein (CP) subunits play vital roles in the life cycles of the RNA bacteriophages, e.g., by allowing translational repression of their replicase cistrons and tagging their own RNA genomes for encapsidation. The CPs of bacteriophages Qbeta and MS2 each discriminate in favor of their cognate translational operators, even in the presence of closely related operators from other phages in vivo. Discrete mutations within the MS2 CP have been shown to relax this discrimination in vitro. We have determined the structures of eight complexes between such mutants and both MS2 and Qbeta stem-loops with X-ray crystallography. In conjunction with previously determined in vivo repression data, the structures enable us to propose the molecular basis for the discrimination mechanism.
About this Structure
2B2D is a Single protein structure of sequence from Enterobacterio phage ms2. Full crystallographic information is available from OCA.
Reference
Structural basis of RNA binding discrimination between bacteriophages Qbeta and MS2., Horn WT, Tars K, Grahn E, Helgstrand C, Baron AJ, Lago H, Adams CJ, Peabody DS, Phillips SE, Stonehouse NJ, Liljas L, Stockley PG, Structure. 2006 Mar;14(3):487-95. PMID:16531233
Page seeded by OCA on Mon Mar 31 02:00:32 2008
Categories: Enterobacterio phage ms2 | Single protein | Adams, C J. | Baron, A J. | Grahn, E. | Helgstrand, C. | Horn, W T. | Lago, H. | Liljas, L. | Peabody, D S. | Phillips, S E.V. | Stockley, P G. | Stonehouse, N J. | Tars, K. | Capsid | Complex (capsid protein - rna hairpin) hairpin | Levivirus | Protein/rna | Virus/viral
