2b5f
From Proteopedia
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|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1z98|1Z98]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2b5f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b5f OCA], [http://www.ebi.ac.uk/pdbsum/2b5f PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2b5f RCSB]</span> | ||
}} | }} | ||
| Line 30: | Line 33: | ||
[[Category: Tornroth-Horsefield, S.]] | [[Category: Tornroth-Horsefield, S.]] | ||
[[Category: Wang, Y.]] | [[Category: Wang, Y.]] | ||
| - | [[Category: aquaporin | + | [[Category: aquaporin]] |
| + | [[Category: major intrinsic protein]] | ||
| + | [[Category: mip]] | ||
| + | [[Category: pip]] | ||
| + | [[Category: plasma membrane intrinsic protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:01:43 2008'' |
Revision as of 23:01, 30 March 2008
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| , resolution 3.9Å | |||||||
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| Related: | 1Z98
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of the spinach aquaporin SoPIP2;1 in an open conformation to 3.9 resolution
Overview
Plants counteract fluctuations in water supply by regulating all aquaporins in the cell plasma membrane. Channel closure results either from the dephosphorylation of two conserved serine residues under conditions of drought stress, or from the protonation of a conserved histidine residue following a drop in cytoplasmic pH due to anoxia during flooding. Here we report the X-ray structure of the spinach plasma membrane aquaporin SoPIP2;1 in its closed conformation at 2.1 A resolution and in its open conformation at 3.9 A resolution, and molecular dynamics simulations of the initial events governing gating. In the closed conformation loop D caps the channel from the cytoplasm and thereby occludes the pore. In the open conformation loop D is displaced up to 16 A and this movement opens a hydrophobic gate blocking the channel entrance from the cytoplasm. These results reveal a molecular gating mechanism which appears conserved throughout all plant plasma membrane aquaporins.
About this Structure
2B5F is a Single protein structure of sequence from Spinacia oleracea. Full crystallographic information is available from OCA.
Reference
Structural mechanism of plant aquaporin gating., Tornroth-Horsefield S, Wang Y, Hedfalk K, Johanson U, Karlsson M, Tajkhorshid E, Neutze R, Kjellbom P, Nature. 2006 Feb 9;439(7077):688-94. Epub 2005 Dec 7. PMID:16340961
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