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4fg6

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Revision as of 14:26, 5 August 2016

Structure of EcCLC E148A mutant in Glutamate

Structural highlights

4fg6 is a 6 chain structure with sequence from Lk3 transgenic mice and Shiss. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	clcA, eriC, SSON_0167 (SHISS)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[CLCA_SHISS] Proton-coupled chloride transporter. Functions as antiport system and exchanges two chloride ions for 1 proton. Probably acts as an electrical shunt for an outwardly-directed proton pump that is linked to amino acid decarboxylation, as part of the extreme acid resistance (XAR) response.[HAMAP-Rule:MF_01128]

Publication Abstract from PubMed

CLC proteins underlie muscle, kidney, bone, and other organ system function by catalyzing the transport of Cl(-) ions across cell and organellar membranes. Some CLC proteins are ion channels while others are pumps that exchange Cl(-) for H(+). The pathway through which Cl(-) ions cross the membrane has been characterized, but the transport of H(+) and the principle by which their movement is coupled to Cl(-) movement is not well understood. Here we show that H(+) transport depends not only on the presence of a specific glutamate residue but also the presence of Cl(-) ions. H(+) transport, however, can be isolated and analyzed in the absence of Cl(-) by mutating the glutamate to alanine and adding carboxylate-containing molecules to solution, consistent with the notion that H(+) transfer is mediated through the entry of a carboxylate group into the anion pathway. Cl(-) ions and carboxylate interact with each other strongly. These data support a mechanism in which the glutamate carboxylate functions as a surrogate Cl(-) ion, but it can accept a H(+) and transfer it between the external solution and the central Cl(-) binding site, coupled to the movement of 2 Cl(-) ions.

Molecular mechanism of proton transport in CLC Cl-/H+ exchange transporters.,Feng L, Campbell EB, Mackinnon R Proc Natl Acad Sci U S A. 2012 Jul 17;109(29):11699-704. Epub 2012 Jul 2. PMID:22753511^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Feng L, Campbell EB, Mackinnon R. Molecular mechanism of proton transport in CLC Cl-/H+ exchange transporters. Proc Natl Acad Sci U S A. 2012 Jul 17;109(29):11699-704. Epub 2012 Jul 2. PMID:22753511 doi:10.1073/pnas.1205764109

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4fg6"

@@ Line 1: / Line 1: @@
 ==Structure of EcCLC E148A mutant in Glutamate==
 <StructureSection load='4fg6' size='340' side='right' caption='[[4fg6]], [[Resolution|resolution]] 3.02&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4fg6]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [http://en.wikipedia.org/wiki/Shigella_sonnei_ss046 Shigella sonnei ss046]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FG6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FG6 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4fg6]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice] and [http://en.wikipedia.org/wiki/Shiss Shiss]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FG6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FG6 FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">clcA, eriC, SSON_0167 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300269 Shigella sonnei Ss046])</td></tr>
+</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">clcA, eriC, SSON_0167 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300269 SHISS])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fg6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fg6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4fg6 RCSB], [http://www.ebi.ac.uk/pdbsum/4fg6 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fg6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fg6 OCA], [http://pdbe.org/4fg6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4fg6 RCSB], [http://www.ebi.ac.uk/pdbsum/4fg6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4fg6 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 16: / Line 17: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4fg6" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Mus musculus]]
+[[Category: Lk3 transgenic mice]]
-[[Category: Shigella sonnei ss046]]
+[[Category: Shiss]]
 [[Category: Feng, L]]
 [[Category: MacKinnon, R]]

4fg6

From Proteopedia

Revision as of 14:26, 5 August 2016

Structure of EcCLC E148A mutant in Glutamate

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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