4owx

From Proteopedia

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Revision as of 17:35, 5 August 2016

Structural basis of SOSS1 in complex with a 12nt ssDNA

Structural highlights

4owx is a 3 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	INTS3, C1orf193, C1orf60 (HUMAN), NABP2, OBFC2B, SSB1, LP3587 (HUMAN)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[SOSB1_HUMAN] Component of the SOSS complex, a multiprotein complex that functions downstream of the MRN complex to promote DNA repair and G2/M checkpoint. In the SOSS complex, acts as a sensor of single-stranded DNA that binds to single-stranded DNA, in particular to polypyrimidines. The SOSS complex associates with DNA lesions and influences diverse endpoints in the cellular DNA damage response including cell-cycle checkpoint activation, recombinational repair and maintenance of genomic stability. Required for efficient homologous recombination-dependent repair of double-strand breaks (DSBs) and ATM-dependent signaling pathways.^[1] ^[2] ^[3] [INT3_HUMAN] Component of the Integrator complex. The Integrator complex is involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes.^[4] ^[5] Component of the SOSS complex, a multiprotein complex that functions downstream of the MRN complex to promote DNA repair and G2/M checkpoint. The SOSS complex associates with single-stranded DNA at DNA lesions and influences diverse endpoints in the cellular DNA damage response including cell-cycle checkpoint activation, recombinational repair and maintenance of genomic stability. The SOSS complex is required for efficient homologous recombination-dependent repair of double-strand breaks (DSBs) and ATM-dependent signaling pathways. In the SOSS complex, it is required for the assembly of the complex and for stabilization of the complex at DNA damage sites.^[6] ^[7]

Publication Abstract from PubMed

The SOSS1 complex comprising SOSSA, SOSSB1, and SOSSC senses single-stranded DNA (ssDNA) and promotes repair of DNA double-strand breaks (DSBs). But how SOSS1 is assembled and recognizes ssDNA remains elusive. The crystal structure of the N-terminal half of SOSSA (SOSSAN) in complex with SOSSB1 and SOSSC showed that SOSSAN serves as a scaffold to bind both SOSSB1 and SOSSC for assembly of the SOSS1 complex. The structures of SOSSAN/B1 in complex with a 12 nt ssDNA and SOSSAN/B1/C in complex with a 35 nt ssDNA showed that SOSSB1 interacts with both SOSSAN and ssDNA via two distinct surfaces. Recognition of ssDNA with a length of up to nine nucleotides is mediated solely by SOSSB1, whereas neither SOSSC nor SOSSAN are critical for ssDNA binding. These results reveal the structural basis of SOSS1 assembly and provide a framework for further study of the mechanism governing longer ssDNA recognition by the SOSS1 complex during DSB repair.

Structural Basis of SOSS1 Complex Assembly and Recognition of ssDNA.,Ren W, Chen H, Sun Q, Tang X, Lim SC, Huang J, Song H Cell Rep. 2014 Mar 27;6(6):982-91. doi: 10.1016/j.celrep.2014.02.020. Epub 2014, Mar 13. PMID:24630995^[8]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Richard DJ, Bolderson E, Cubeddu L, Wadsworth RI, Savage K, Sharma GG, Nicolette ML, Tsvetanov S, McIlwraith MJ, Pandita RK, Takeda S, Hay RT, Gautier J, West SC, Paull TT, Pandita TK, White MF, Khanna KK. Single-stranded DNA-binding protein hSSB1 is critical for genomic stability. Nature. 2008 May 29;453(7195):677-81. doi: 10.1038/nature06883. Epub 2008 Apr 30. PMID:18449195 doi:http://dx.doi.org/10.1038/nature06883
↑ Li Y, Bolderson E, Kumar R, Muniandy PA, Xue Y, Richard DJ, Seidman M, Pandita TK, Khanna KK, Wang W. HSSB1 and hSSB2 form similar multiprotein complexes that participate in DNA damage response. J Biol Chem. 2009 Aug 28;284(35):23525-31. doi: 10.1074/jbc.C109.039586. Epub, 2009 Jul 14. PMID:19605351 doi:http://dx.doi.org/10.1074/jbc.C109.039586
↑ Huang J, Gong Z, Ghosal G, Chen J. SOSS complexes participate in the maintenance of genomic stability. Mol Cell. 2009 Aug 14;35(3):384-93. doi: 10.1016/j.molcel.2009.06.011. PMID:19683501 doi:http://dx.doi.org/10.1016/j.molcel.2009.06.011
↑ Li Y, Bolderson E, Kumar R, Muniandy PA, Xue Y, Richard DJ, Seidman M, Pandita TK, Khanna KK, Wang W. HSSB1 and hSSB2 form similar multiprotein complexes that participate in DNA damage response. J Biol Chem. 2009 Aug 28;284(35):23525-31. doi: 10.1074/jbc.C109.039586. Epub, 2009 Jul 14. PMID:19605351 doi:http://dx.doi.org/10.1074/jbc.C109.039586
↑ Huang J, Gong Z, Ghosal G, Chen J. SOSS complexes participate in the maintenance of genomic stability. Mol Cell. 2009 Aug 14;35(3):384-93. doi: 10.1016/j.molcel.2009.06.011. PMID:19683501 doi:http://dx.doi.org/10.1016/j.molcel.2009.06.011
↑ Li Y, Bolderson E, Kumar R, Muniandy PA, Xue Y, Richard DJ, Seidman M, Pandita TK, Khanna KK, Wang W. HSSB1 and hSSB2 form similar multiprotein complexes that participate in DNA damage response. J Biol Chem. 2009 Aug 28;284(35):23525-31. doi: 10.1074/jbc.C109.039586. Epub, 2009 Jul 14. PMID:19605351 doi:http://dx.doi.org/10.1074/jbc.C109.039586
↑ Huang J, Gong Z, Ghosal G, Chen J. SOSS complexes participate in the maintenance of genomic stability. Mol Cell. 2009 Aug 14;35(3):384-93. doi: 10.1016/j.molcel.2009.06.011. PMID:19683501 doi:http://dx.doi.org/10.1016/j.molcel.2009.06.011
↑ Ren W, Chen H, Sun Q, Tang X, Lim SC, Huang J, Song H. Structural Basis of SOSS1 Complex Assembly and Recognition of ssDNA. Cell Rep. 2014 Mar 27;6(6):982-91. doi: 10.1016/j.celrep.2014.02.020. Epub 2014, Mar 13. PMID:24630995 doi:http://dx.doi.org/10.1016/j.celrep.2014.02.020

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4owx"

@@ Line 1: / Line 1: @@
 ==Structural basis of SOSS1 in complex with a 12nt ssDNA==
 <StructureSection load='4owx' size='340' side='right' caption='[[4owx]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
@@ Line 4: / Line 5: @@
 <table><tr><td colspan='2'>[[4owx]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OWX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OWX FirstGlance]. <br>
 </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">INTS3, C1orf193, C1orf60 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), NABP2, OBFC2B, SSB1, LP3587 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4owx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4owx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4owx RCSB], [http://www.ebi.ac.uk/pdbsum/4owx PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4owx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4owx OCA], [http://pdbe.org/4owx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4owx RCSB], [http://www.ebi.ac.uk/pdbsum/4owx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4owx ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 16: / Line 17: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4owx" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>

4owx

From Proteopedia

Revision as of 17:35, 5 August 2016

Structural basis of SOSS1 in complex with a 12nt ssDNA

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

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