2bow
From Proteopedia
| Line 4: | Line 4: | ||
|PDB= 2bow |SIZE=350|CAPTION= <scene name='initialview01'>2bow</scene>, resolution 2.8Å | |PDB= 2bow |SIZE=350|CAPTION= <scene name='initialview01'>2bow</scene>, resolution 2.8Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=P4P:TETRAPHENYLPHOSPHONIUM'>P4P</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bow FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bow OCA], [http://www.ebi.ac.uk/pdbsum/2bow PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2bow RCSB]</span> | ||
}} | }} | ||
| Line 26: | Line 29: | ||
[[Category: Neyfakh, A A.]] | [[Category: Neyfakh, A A.]] | ||
[[Category: Zheleznova, E E.]] | [[Category: Zheleznova, E E.]] | ||
| - | [[Category: MN]] | ||
| - | [[Category: P4P]] | ||
| - | [[Category: SO4]] | ||
[[Category: multidrug binding]] | [[Category: multidrug binding]] | ||
[[Category: transcription activator]] | [[Category: transcription activator]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:09:35 2008'' |
Revision as of 23:09, 30 March 2008
| |||||||
| , resolution 2.8Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
MULTIDRUG-BINDING DOMAIN OF TRANSCRIPTION ACTIVATOR BMRR IN COMPLEX WITH A LIGAND, TETRAPHENYLPHOSPHONIUM
Overview
Multidrug-efflux transporters demonstrate an unusual ability to recognize multiple structurally dissimilar toxins. A comparable ability to bind diverse hydrophobic cationic drugs is characteristic of the Bacillus subtilis transcription regulator BmrR, which upon drug binding activates expression of the multidrug transporter Bmr. Crystal structures of the multidrug-binding domain of BmrR (2.7 A resolution) and of its complex with the drug tetraphenylphosphonium (2.8 A resolution) revealed a drug-induced unfolding and relocation of an alpha helix, which exposes an internal drug-binding pocket. Tetraphenylphosphonium binding is mediated by stacking and van der Waals contacts with multiple hydrophobic residues of the pocket and by an electrostatic interaction between the positively charged drug and a buried glutamate residue, which is the key to cation selectivity. Similar binding principles may be used by other multidrug-binding proteins.
About this Structure
2BOW is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.
Reference
Structural basis of multidrug recognition by BmrR, a transcription activator of a multidrug transporter., Zheleznova EE, Markham PN, Neyfakh AA, Brennan RG, Cell. 1999 Feb 5;96(3):353-62. PMID:10025401
Page seeded by OCA on Mon Mar 31 02:09:35 2008
