1o6f
From Proteopedia
(New page: 200px<br /> <applet load="1o6f" size="450" color="white" frame="true" align="right" spinBox="true" caption="1o6f, resolution 1.6Å" /> '''PROLYL OLIGOPEPTIDAS...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1O6F is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/ ]] with SIN and GOL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.26 3.4.21.26]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1O6F OCA]]. | + | 1O6F is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/ ]] with SIN and GOL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Prolyl_oligopeptidase Prolyl oligopeptidase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.26 3.4.21.26]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1O6F OCA]]. |
==Reference== | ==Reference== | ||
Substrate-dependent competency of the catalytic triad of prolyl oligopeptidase., Szeltner Z, Rea D, Juhasz T, Renner V, Mucsi Z, Orosz G, Fulop V, Polgar L, J Biol Chem. 2002 Nov 22;277(47):44597-605. Epub 2002 Sep 11. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12228249 12228249] | Substrate-dependent competency of the catalytic triad of prolyl oligopeptidase., Szeltner Z, Rea D, Juhasz T, Renner V, Mucsi Z, Orosz G, Fulop V, Polgar L, J Biol Chem. 2002 Nov 22;277(47):44597-605. Epub 2002 Sep 11. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12228249 12228249] | ||
| + | [[Category: Prolyl oligopeptidase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Fulop, V.]] | [[Category: Fulop, V.]] | ||
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[[Category: prolyl oligopeptidase]] | [[Category: prolyl oligopeptidase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 10:52:06 2007'' |
Revision as of 08:47, 30 October 2007
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PROLYL OLIGOPEPTIDASE FROM PORCINE BRAIN, D641A MUTANT WITH BOUND PEPTIDE LIGAND SUC-GLY-PRO
Overview
Prolyl oligopeptidase, a serine peptidase unrelated to trypsin and, subtilisin, is implicated in memory disorders and is an important target, of drug design. The catalytic competence of the Asp(641) residue of the, catalytic triad (Ser(554), Asp(641), His(680)) was studied using the D641N, and D641A variants of the enzyme. Both variants displayed 3 orders of, magnitude reduction in k(cat)/K(m) for, benzyloxycarbonyl-Gly-Pro-2-naphthylamide. Using an octapeptide substrate, the decrease was 6 orders of magnitude, whereas with, Z-Gly-Pro-4-nitrophenyl ester there was virtually no change in, k(cat)/K(m). This indicates that the contribution of Asp(641) is very much, dependent on the substrate-leaving group, which was not the case for the, classic serine peptidase, trypsin. The rate constant ... [(full description)]
About this Structure
1O6F is a [Single protein] structure of sequence from [[1]] with SIN and GOL as [ligands]. Active as [Prolyl oligopeptidase], with EC number [3.4.21.26]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Substrate-dependent competency of the catalytic triad of prolyl oligopeptidase., Szeltner Z, Rea D, Juhasz T, Renner V, Mucsi Z, Orosz G, Fulop V, Polgar L, J Biol Chem. 2002 Nov 22;277(47):44597-605. Epub 2002 Sep 11. PMID:12228249
Page seeded by OCA on Tue Oct 30 10:52:06 2007
