2btj
From Proteopedia
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|PDB= 2btj |SIZE=350|CAPTION= <scene name='initialview01'>2btj</scene>, resolution 2.0Å | |PDB= 2btj |SIZE=350|CAPTION= <scene name='initialview01'>2btj</scene>, resolution 2.0Å | ||
|SITE= <scene name='pdbsite=AC1:Iey+Binding+Site+For+Chain+D'>AC1</scene> | |SITE= <scene name='pdbsite=AC1:Iey+Binding+Site+For+Chain+D'>AC1</scene> | ||
| - | |LIGAND= <scene name='pdbligand=IEY:2-((1E)-2-(5-IMIDAZOLYL)ETHENYL)-4-(P-HYDROXYBENZYLIDENE)-5-IMIDAZOLINONE'>IEY</scene> | + | |LIGAND= <scene name='pdbligand=IEY:2-((1E)-2-(5-IMIDAZOLYL)ETHENYL)-4-(P-HYDROXYBENZYLIDENE)-5-IMIDAZOLINONE'>IEY</scene>, <scene name='pdbligand=NFA:PHENYLALANINE+AMIDE'>NFA</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2btj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2btj OCA], [http://www.ebi.ac.uk/pdbsum/2btj PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2btj RCSB]</span> | ||
}} | }} | ||
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[[Category: Nienhaus, K.]] | [[Category: Nienhaus, K.]] | ||
[[Category: Wiedenmann, J.]] | [[Category: Wiedenmann, J.]] | ||
| - | [[Category: IEY]] | ||
[[Category: fluorescent protein]] | [[Category: fluorescent protein]] | ||
[[Category: green-to-red conversion]] | [[Category: green-to-red conversion]] | ||
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[[Category: photo-induced protein cleavage]] | [[Category: photo-induced protein cleavage]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:11:33 2008'' |
Revision as of 23:11, 30 March 2008
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| , resolution 2.0Å | |||||||
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| Ligands: | , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
FLUORESCENT PROTEIN EOSFP- RED FORM
Overview
Genetically encoded fusion constructs derived from fluorescent proteins (FPs) can be designed to report on a multitude of events and signals in cells, tissues, and entire organs without interfering with the complex machinery of life. EosFP is a novel FP from the scleractinian coral Lobophyllia hemprichii that switches its fluorescence emission from green (516 nm) to red (581 nm) upon irradiation with approximately 400-nm light. This property enables localized tagging of proteins and thus provides a valuable tool for tracking protein movements within live cells. Here, we present the x-ray structures of the green and red forms of WT EosFP. They reveal that formation of the red chromophore is associated with cleavage of the peptide backbone, with surprisingly little change elsewhere in the structure, and provide insights into the mechanism that generates this interesting posttranslational polypeptide modification.
About this Structure
2BTJ is a Single protein structure of sequence from Lobophyllia hemprichii. Full crystallographic information is available from OCA.
Reference
Structural basis for photo-induced protein cleavage and green-to-red conversion of fluorescent protein EosFP., Nienhaus K, Nienhaus GU, Wiedenmann J, Nar H, Proc Natl Acad Sci U S A. 2005 Jun 28;102(26):9156-9. Epub 2005 Jun 17. PMID:15964985
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