2bzx
From Proteopedia
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|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bzx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bzx OCA], [http://www.ebi.ac.uk/pdbsum/2bzx PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2bzx RCSB]</span> | ||
}} | }} | ||
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[[Category: Harkiolaki, M.]] | [[Category: Harkiolaki, M.]] | ||
[[Category: Jones, E Y.]] | [[Category: Jones, E Y.]] | ||
| - | [[Category: crkl | + | [[Category: crkl,sh3c,monomer,native,nuclear export]] |
| - | + | ||
| - | + | ||
| - | + | ||
[[Category: sh3 domain]] | [[Category: sh3 domain]] | ||
| - | [[Category: sh3c]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:14:18 2008'' |
Revision as of 23:14, 30 March 2008
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| , resolution 2.8Å | |||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
ATOMIC MODEL OF CRKL-SH3C MONOMER
Overview
CRKL plays essential roles in cell signaling. It consists of an N-terminal SH2 domain followed by two SH3 domains. SH2 and SH3N bind to signaling proteins, but the function of the SH3C domain has remained largely enigmatic. We show here that the SH3C of CRKL forms homodimers in protein crystals and in solution. Evidence for dimer formation of full-length CRKL is also presented. In the SH3C dimer, a nuclear export signal (NES) is mostly buried under the domain surface. The same is true for a monomeric SH3C obtained under different crystallization conditions. Interestingly, partial SH3 unfolding, such as occurs upon dimer/monomer transition, produces a fully-accessible NES through translocation of a single beta strand. Our results document the existence of an SH3 domain dimer formed through exchange of the first SH3 domain beta strand and suggest that partial unfolding of the SH3C is important for the relay of information in vivo.
About this Structure
2BZX is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
The C-terminal SH3 domain of CRKL as a dynamic dimerization module transiently exposing a nuclear export signal., Harkiolaki M, Gilbert RJ, Jones EY, Feller SM, Structure. 2006 Dec;14(12):1741-53. PMID:17161365
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