2c4b

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|PDB= 2c4b |SIZE=350|CAPTION= <scene name='initialview01'>2c4b</scene>, resolution 1.30&Aring;
|PDB= 2c4b |SIZE=350|CAPTION= <scene name='initialview01'>2c4b</scene>, resolution 1.30&Aring;
|SITE= <scene name='pdbsite=AC1:So4+Binding+Site+For+Chain+B'>AC1</scene>
|SITE= <scene name='pdbsite=AC1:So4+Binding+Site+For+Chain+B'>AC1</scene>
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|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=2PE:NONAETHYLENE+GLYCOL'>2PE</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=UNX:UNKNOWN+ATOM+OR+ION'>UNX</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene> and <scene name='pdbligand=FMT:FORMIC ACID'>FMT</scene>
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|LIGAND= <scene name='pdbligand=2PE:NONAETHYLENE+GLYCOL'>2PE</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=UNX:UNKNOWN+ATOM+OR+ION'>UNX</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Ribonuclease_T(1) Ribonuclease T(1)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.3 3.1.27.3]
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_T(1) Ribonuclease T(1)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.3 3.1.27.3] </span>
|GENE=
|GENE=
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|DOMAIN=
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2c4b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c4b OCA], [http://www.ebi.ac.uk/pdbsum/2c4b PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2c4b RCSB]</span>
}}
}}
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[[Category: Schmoldt, H U.]]
[[Category: Schmoldt, H U.]]
[[Category: Wentzel, A.]]
[[Category: Wentzel, A.]]
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[[Category: 2PE]]
 
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[[Category: EDO]]
 
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[[Category: FMT]]
 
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[[Category: GOL]]
 
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[[Category: MES]]
 
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[[Category: SO4]]
 
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[[Category: UNX]]
 
[[Category: endonuclease]]
[[Category: endonuclease]]
[[Category: fusion protein]]
[[Category: fusion protein]]
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[[Category: squash inhibitor]]
[[Category: squash inhibitor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:10:36 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:16:04 2008''

Revision as of 23:16, 30 March 2008

Image:2c4b.gif


PDB ID 2c4b

Drag the structure with the mouse to rotate
, resolution 1.30Å
Sites:
Ligands: , , , , , ,
Activity: Ribonuclease T(1), with EC number 3.1.27.3
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



INHIBITOR CYSTINE KNOT PROTEIN MCOEETI FUSED TO THE CATALYTICALLY INACTIVE BARNASE MUTANT H102A


Overview

We present a fusion system suited to determine the crystal structure of small disulfide-rich proteins. McoEeTI, a hybrid inhibitor cystine knot microprotein, was produced as a soluble fusion to a catalytically inactive variant of the RNAse barnase in Escherichia coli. Functioning as a versatile tag, barnase facilitated purification, crystallization and high-resolution structure determination. Flexibility of the linker region allows for different relative orientations of barnase and the fusion partner in two crystallographically independent molecules and may thereby facilitate crystal packing. Nevertheless, the linker region is well ordered in both molecules. This system may prove more generally useful to determine the crystal structure of peptides and small proteins.

About this Structure

2C4B is a Single protein structure of sequence from Bacillus amyloliquefaciens, momordica cochinchinensis, ecballium elaterium. Full crystallographic information is available from OCA.

Reference

Barnase fusion as a tool to determine the crystal structure of the small disulfide-rich protein McoEeTI., Niemann HH, Schmoldt HU, Wentzel A, Kolmar H, Heinz DW, J Mol Biol. 2006 Feb 10;356(1):1-8. Epub 2005 Nov 21. PMID:16337652

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