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2c5s

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|PDB= 2c5s |SIZE=350|CAPTION= <scene name='initialview01'>2c5s</scene>, resolution 2.50&Aring;
|PDB= 2c5s |SIZE=350|CAPTION= <scene name='initialview01'>2c5s</scene>, resolution 2.50&Aring;
|SITE= <scene name='pdbsite=AC1:Amp+Binding+Site+For+Chain+A'>AC1</scene>
|SITE= <scene name='pdbsite=AC1:Amp+Binding+Site+For+Chain+A'>AC1</scene>
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|LIGAND= <scene name='pdbligand=AMP:ADENOSINE MONOPHOSPHATE'>AMP</scene>
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|LIGAND= <scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>
|ACTIVITY=
|ACTIVITY=
|GENE=
|GENE=
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|DOMAIN=
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2c5s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c5s OCA], [http://www.ebi.ac.uk/pdbsum/2c5s PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2c5s RCSB]</span>
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}}
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[[Category: Ortiz-Lombardia, M.]]
[[Category: Ortiz-Lombardia, M.]]
[[Category: Waterman, D G.]]
[[Category: Waterman, D G.]]
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[[Category: AMP]]
 
[[Category: 4-thiouridine synthase]]
[[Category: 4-thiouridine synthase]]
[[Category: ferredoxin-like domain]]
[[Category: ferredoxin-like domain]]
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[[Category: trna modification]]
[[Category: trna modification]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:11:11 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:16:46 2008''

Revision as of 23:16, 30 March 2008

Image:2c5s.gif


PDB ID 2c5s

Drag the structure with the mouse to rotate
, resolution 2.50Å
Sites:
Ligands:
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



CRYSTAL STRUCTURE OF BACILLUS ANTHRACIS THII, A TRNA-MODIFYING ENZYME CONTAINING THE PREDICTED RNA-BINDING THUMP DOMAIN


Overview

ThiI is an enzyme responsible for the formation of the modified base S(4)U (4-thiouridine) found at position 8 in some prokaryotic tRNAs. This base acts as a sensitive trigger for the response mechanism to UV exposure, providing protection against its damaging effects. We present the crystal structure of Bacillus anthracis ThiI in complex with AMP, revealing an extended tripartite architecture in which an N-terminal ferredoxin-like domain (NFLD) connects the C-terminal catalytic PP-loop pyrophosphatase domain with a THUMP domain, an ancient predicted RNA-binding domain that is widespread in all kingdoms of life. We describe the structure of the THUMP domain, which appears to be unrelated to RNA-binding domains of known structure. Mapping the conserved residues of NFLD and the THUMP domain onto the ThiI structure suggests that these domains jointly form the tRNA-binding surface. The inaccessibility of U8 in the canonical L-shaped form of tRNA, and the existence of a glycine-rich linker joining the catalytic and RNA-binding moieties of ThiI suggest that structural changes may occur in both molecules upon binding.

About this Structure

2C5S is a Single protein structure of sequence from Bacillus anthracis. Full crystallographic information is available from OCA.

Reference

Crystal structure of Bacillus anthracis ThiI, a tRNA-modifying enzyme containing the predicted RNA-binding THUMP domain., Waterman DG, Ortiz-Lombardia M, Fogg MJ, Koonin EV, Antson AA, J Mol Biol. 2006 Feb 10;356(1):97-110. Epub 2005 Nov 22. PMID:16343540

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