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3of1

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Revision as of 09:01, 4 August 2016

Crystal Structure of Bcy1, the Yeast Regulatory Subunit of PKA

Structural highlights

3of1 is a 1 chain structure with sequence from Atcc 18824. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	1rgs, 1cx4
Gene:	BCY1, REG1, SRA1, YIL033C (ATCC 18824)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[KAPR_YEAST] Regulatory subunit of the cyclic AMP-dependent protein kinase (PKA), an effector of the Ras/cAMP pathway. Inhibits PKA activity in the absence of cAMP. cAMP activates PKA and promotes growth and proliferation in response to good nutrient conditions. Together with ZDS1, provides a negative feedback control on the cell wall integrity-signaling pathway by acting as a negative regulator of MAP kinase SLT2/MPK1.^[1] ^[2] ^[3] ^[4]

Publication Abstract from PubMed

The major cAMP receptors in eukaryotes are the regulatory (R) subunits of PKA, an allosteric enzyme conserved in fungi through mammals. While mammals have four R-subunit genes, Saccharomyces cerevisiae has only one, Bcy1. To achieve a molecular understanding of PKA activation in yeast and to explore the evolution of cyclic-nucleotide binding (CNB) domains, we solved the structure of cAMP-bound Bcy1(168-416). Surprisingly, the relative orientation of the two CNB domains in Bcy1 is very different from mammalian R-subunits. This quaternary structure is defined primarily by a fungi-specific sequence in the hinge between the alphaB/alphaC helices of the CNB-A domain. The unique interface between the two CNB domains in Bcy1 defines the allosteric mechanism for cooperative activation of PKA by cAMP. Some interface motifs are isoform-specific while others, although conserved, play surprisingly different roles in each R-subunit. Phylogenetic analysis shows that structural differences in Bcy1 are shared by fungi of the subphylum Saccharomycotina.

Structure of yeast regulatory subunit: a glimpse into the evolution of PKA signaling.,Rinaldi J, Wu J, Yang J, Ralston CY, Sankaran B, Moreno S, Taylor SS Structure. 2010 Nov 10;18(11):1471-82. PMID:21070946^[5]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Toda T, Cameron S, Sass P, Zoller M, Scott JD, McMullen B, Hurwitz M, Krebs EG, Wigler M. Cloning and characterization of BCY1, a locus encoding a regulatory subunit of the cyclic AMP-dependent protein kinase in Saccharomyces cerevisiae. Mol Cell Biol. 1987 Apr;7(4):1371-7. PMID:3037314
↑ Cannon JF, Tatchell K. Characterization of Saccharomyces cerevisiae genes encoding subunits of cyclic AMP-dependent protein kinase. Mol Cell Biol. 1987 Aug;7(8):2653-63. PMID:2823100
↑ Hixson CS, Krebs EG. Characterization of a cyclic AMP-binding protein from bakers' yeast. Identification as a regulatory subunit of cyclic AMP-dependent protein kinase. J Biol Chem. 1980 Mar 10;255(5):2137-45. PMID:6243658
↑ Griffioen G, Swinnen S, Thevelein JM. Feedback inhibition on cell wall integrity signaling by Zds1 involves Gsk3 phosphorylation of a cAMP-dependent protein kinase regulatory subunit. J Biol Chem. 2003 Jun 27;278(26):23460-71. Epub 2003 Apr 18. PMID:12704202 doi:10.1074/jbc.M210691200
↑ Rinaldi J, Wu J, Yang J, Ralston CY, Sankaran B, Moreno S, Taylor SS. Structure of yeast regulatory subunit: a glimpse into the evolution of PKA signaling. Structure. 2010 Nov 10;18(11):1471-82. PMID:21070946 doi:10.1016/j.str.2010.08.013

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3of1"

@@ Line 1: / Line 1: @@
 ==Crystal Structure of Bcy1, the Yeast Regulatory Subunit of PKA==
 <StructureSection load='3of1' size='340' side='right' caption='[[3of1]], [[Resolution|resolution]] 2.21&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3of1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OF1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3OF1 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3of1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OF1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3OF1 FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CMP:ADENOSINE-3,5-CYCLIC-MONOPHOSPHATE'>CMP</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1rgs|1rgs]], [[1cx4|1cx4]]</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BCY1, REG1, SRA1, YIL033C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BCY1, REG1, SRA1, YIL033C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3of1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3of1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3of1 RCSB], [http://www.ebi.ac.uk/pdbsum/3of1 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3of1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3of1 OCA], [http://pdbe.org/3of1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3of1 RCSB], [http://www.ebi.ac.uk/pdbsum/3of1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3of1 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 18: / Line 19: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 3of1" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[CAMP-dependent protein kinase|CAMP-dependent protein kinase]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Saccharomyces cerevisiae]]
+[[Category: Atcc 18824]]
 [[Category: Moreno, S]]
 [[Category: Ralston, C Y]]

3of1

From Proteopedia

Revision as of 09:01, 4 August 2016

Crystal Structure of Bcy1, the Yeast Regulatory Subunit of PKA

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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