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3r65

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Revision as of 10:30, 4 August 2016

MthK channel pore E92Q mutant

Structural highlights

3r65 is a 1 chain structure with sequence from Metth. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	3ldc
Gene:	mthK, MTH_1520 (METTH)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[MTHK_METTH] Calcium-gated potassium channel.

Publication Abstract from PubMed

While selective for K, K channels vary significantly among their rate of ion permeation. Here, we probe the effect of steric hindrance and electrostatics within the ion conduction pathway on K permeation in the MthK K channel using structure-based mutagenesis combined with single-channel electrophysiology and X-ray crystallography. We demonstrate that changes in side-chain size and polarity at Ala88, which forms the constriction point of the open MthK pore, have profound effects on single-channel conductance as well as open probability. We also reveal that the negatively charged Glu92s at the intracellular entrance of the open pore form an electrostatic trap, which stabilizes a hydrated K and facilitates ion permeation. This electrostatic attraction is also responsible for intracellular divalent blockage, which renders the channel inward rectified in the presence of Ca(2). In light of the high structural conservation of the selectivity filter, the size and chemical environment differences within the portion of the ion conduction pathway other than the filter are likely the determinants for the conductance variations among K channels.

Crucial points within the pore as determinants of K channel conductance and gating.,Shi N, Zeng W, Ye S, Li Y, Jiang Y J Mol Biol. 2011 Aug 5;411(1):27-35. Epub 2011 Apr 30. PMID:21554888^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Shi N, Zeng W, Ye S, Li Y, Jiang Y. Crucial points within the pore as determinants of K channel conductance and gating. J Mol Biol. 2011 Aug 5;411(1):27-35. Epub 2011 Apr 30. PMID:21554888 doi:10.1016/j.jmb.2011.04.058

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3r65"

@@ Line 1: / Line 1: @@
 ==MthK channel pore E92Q mutant==
 <StructureSection load='3r65' size='340' side='right' caption='[[3r65]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3r65]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Methanothermobacter_thermautotrophicus_str._delta_h Methanothermobacter thermautotrophicus str. delta h]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R65 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3R65 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3r65]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Metth Metth]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R65 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3R65 FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3ldc|3ldc]]</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mthK, MTH_1520 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=187420 Methanothermobacter thermautotrophicus str. Delta H])</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mthK, MTH_1520 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=187420 METTH])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3r65 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3r65 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3r65 RCSB], [http://www.ebi.ac.uk/pdbsum/3r65 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3r65 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3r65 OCA], [http://pdbe.org/3r65 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3r65 RCSB], [http://www.ebi.ac.uk/pdbsum/3r65 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3r65 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 18: / Line 19: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 3r65" style="background-color:#fffaf0;"></div>
 ==See Also==
@@ Line 25: / Line 27: @@
 __TOC__
 </StructureSection>
-[[Category: Methanothermobacter thermautotrophicus str. delta h]]
+[[Category: Metth]]
 [[Category: Jiang, Y]]
 [[Category: Li, Y]]

3r65

From Proteopedia

Revision as of 10:30, 4 August 2016

MthK channel pore E92Q mutant

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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