2cl5
From Proteopedia
| Line 4: | Line 4: | ||
|PDB= 2cl5 |SIZE=350|CAPTION= <scene name='initialview01'>2cl5</scene>, resolution 1.60Å | |PDB= 2cl5 |SIZE=350|CAPTION= <scene name='initialview01'>2cl5</scene>, resolution 1.60Å | ||
|SITE= <scene name='pdbsite=AC1:Bu3+Binding+Site+For+Chain+A'>AC1</scene> | |SITE= <scene name='pdbsite=AC1:Bu3+Binding+Site+For+Chain+A'>AC1</scene> | ||
| - | |LIGAND= | + | |LIGAND= <scene name='pdbligand=BIE:(3,4-DIHYDROXY-2-NITROPHENYL)(PHENYL)METHANONE'>BIE</scene>, <scene name='pdbligand=BU3:(R,R)-2,3-BUTANEDIOL'>BU3</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Catechol_O-methyltransferase Catechol O-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.6 2.1.1.6] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Catechol_O-methyltransferase Catechol O-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.6 2.1.1.6] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cl5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cl5 OCA], [http://www.ebi.ac.uk/pdbsum/2cl5 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2cl5 RCSB]</span> | ||
}} | }} | ||
| Line 31: | Line 34: | ||
[[Category: Rodrigues, M L.]] | [[Category: Rodrigues, M L.]] | ||
[[Category: Soares-Da-Silva, P.]] | [[Category: Soares-Da-Silva, P.]] | ||
| - | [[Category: BIE]] | ||
| - | [[Category: BU3]] | ||
| - | [[Category: MES]] | ||
| - | [[Category: MG]] | ||
| - | [[Category: SAM]] | ||
[[Category: alternative initiation]] | [[Category: alternative initiation]] | ||
[[Category: catechol-o-methyltransferase]] | [[Category: catechol-o-methyltransferase]] | ||
| Line 50: | Line 48: | ||
[[Category: transmembrane]] | [[Category: transmembrane]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:23:06 2008'' |
Revision as of 23:23, 30 March 2008
| |||||||
| , resolution 1.60Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | |||||||
| Ligands: | , , , , | ||||||
| Activity: | Catechol O-methyltransferase, with EC number 2.1.1.6 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CATECHOL-O-METHYLTRANSFERASE IN COMPLEX WITH AN INHIBITOR
Overview
In this work, we present a comparative case study of "ortho-" and "meta-nitrated" catecholic inhibitors of catechol-O-methyltransferase (COMT), with regard to their interaction with the catalytic site of the enzyme and the in vitro regioselective formation of their mono-O-methyl ether metabolites. In particular, the effects of altering the attachment position of the inhibitors' side-chain substituent, within the classic nitrocatechol pharmacophore, were investigated. For this purpose, we compared two simple regioisomeric nitrocatechol-type inhibitors of COMT, BIA 3-228 and BIA 8-176, which contain the benzoyl substituent attached at the meta and ortho positions, respectively, relative to the nitro group. The two compounds were slowly O-methylated by COMT in vitro, but the particular substitution pattern of each compound was shown to have a profound impact on the regioselectivity of their O-methylation. To provide a plausible interpretation of these results, a comprehensive analysis of the protein-inhibitor interactions and of the relative chemical susceptibility to O-methylation of the catechol hydroxyl groups was performed by means of docking simulations and ab initio molecular orbital calculations. The major structural and chemical factors that determine the enzyme regioselectivity of O-methylation were identified, and the X-ray structure of the complex of COMT with S-adenosyl-l-methionine and BIA 8-176 is herein disclosed. This is the first reported structure of the soluble form of COMT complexed with a nitrocatecholic inhibitor having a bulky substituent group in adjacent position (ortho) to the nitro group. Structural and dynamic aspects of this complex are analyzed and discussed, in the context of the present study.
About this Structure
2CL5 is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.
Reference
Comparative study of ortho- and meta-nitrated inhibitors of catechol-O-methyltransferase: interactions with the active site and regioselectivity of O-methylation., Palma PN, Rodrigues ML, Archer M, Bonifacio MJ, Loureiro AI, Learmonth DA, Carrondo MA, Soares-da-Silva P, Mol Pharmacol. 2006 Jul;70(1):143-53. Epub 2006 Apr 17. PMID:16618795
Page seeded by OCA on Mon Mar 31 02:23:06 2008
Categories: Catechol O-methyltransferase | Rattus norvegicus | Single protein | Archer, M. | Bonifacio, M J. | Carrondo, M A. | Learmonth, D. | Loureiro, A I. | Palma, P N. | Rodrigues, M L. | Soares-Da-Silva, P. | Alternative initiation | Catechol-o-methyltransferase | Catecholamine metabolism | Comt inhibitor | Magnesium | Membrane | Metal-binding | Methyltransferase | Neurotransmitter degradation | Phosphorylation | Signal-anchor | Transferase | Transmembrane
