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2yn0

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Revision as of 13:44, 4 August 2016

tau55 histidine phosphatase domain

Structural highlights

2yn0 is a 1 chain structure with sequence from Baker's yeast. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[TFC7_YEAST] TFIIIC mediates tRNA and 5S RNA gene activation by binding to intragenic promoter elements. Upstream of the transcription start site, TFIIIC assembles the initiation complex TFIIIB-TFIIIC-tDNA, which is sufficient for RNA polymerase III recruitment and function. Part of the tauA domain of TFIIIC that binds boxA DNA promoter sites of tRNA and similar genes.^[1]

Publication Abstract from PubMed

Saccharomyces cerevisiae tau55, a subunit of the RNA polymerase III-specific general transcription factor TFIIIC, comprises an N-terminal histidine phosphatase domain (tau55-HPD) whose catalytic activity and cellular function is poorly understood. We solved the crystal structures of tau55-HPD and its closely related paralogue Huf and used in silico docking methods to identify phospho-serine and phospho-tyrosine containing peptides as possible substrates that were subsequently validated using in vitro phosphatase assays. A comparative phospho-proteomic study identified additional phosphopeptides as possible targets, which show the involvement of these two phosphatases in the regulation of a variety of cellular functions. Our results identify tau55-HPD and Huf as bona fide protein phosphatases, characterize their substrate specificities and provide a small set of regulated phosphosite targets in vivo.

Structural and functional characterization of a phosphatase domain within yeast general transcription factor TFIIIC.,Taylor NM, Glatt S, Hennrich ML, von Scheven G, Grotsch H, Fernandez-Tornero C, Rybin V, Gavin AC, Kolb P, Muller CW J Biol Chem. 2013 Apr 8. PMID:23569204^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Manaud N, Arrebola R, Buffin-Meyer B, Lefebvre O, Voss H, Riva M, Conesa C, Sentenac A. A chimeric subunit of yeast transcription factor IIIC forms a subcomplex with tau95. Mol Cell Biol. 1998 Jun;18(6):3191-200. PMID:9584160
↑ Taylor NM, Glatt S, Hennrich ML, von Scheven G, Grotsch H, Fernandez-Tornero C, Rybin V, Gavin AC, Kolb P, Muller CW. Structural and functional characterization of a phosphatase domain within yeast general transcription factor TFIIIC. J Biol Chem. 2013 Apr 8. PMID:23569204 doi:10.1074/jbc.M112.427856

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2yn0"

@@ Line 1: / Line 1: @@
 ==tau55 histidine phosphatase domain==
 <StructureSection load='2yn0' size='340' side='right' caption='[[2yn0]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2yn0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_s288c Saccharomyces cerevisiae s288c]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YN0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2YN0 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2yn0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YN0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2YN0 FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2yn0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yn0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2yn0 RCSB], [http://www.ebi.ac.uk/pdbsum/2yn0 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2yn0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yn0 OCA], [http://pdbe.org/2yn0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2yn0 RCSB], [http://www.ebi.ac.uk/pdbsum/2yn0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2yn0 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 16: / Line 17: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 2yn0" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Saccharomyces cerevisiae s288c]]
+[[Category: Baker's yeast]]
 [[Category: Fernandez-Tornero, C]]
 [[Category: Gavin, A C]]

2yn0

From Proteopedia

Revision as of 13:44, 4 August 2016

tau55 histidine phosphatase domain

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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