This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

3ayh

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Revision as of 14:34, 4 August 2016

Crystal structure of the C17/25 subcomplex from S. pombe RNA Polymerase III

Structural highlights

3ayh is a 2 chain structure with sequence from Fission yeast. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Gene:	rpc17, SPAPB1E7.10 (Fission yeast), rpc25, SPBC2G5.07c (Fission yeast)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[RPC9_SCHPO] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs.^[1] [RPC8_SCHPO] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNA.^[2] ^[3]

Publication Abstract from PubMed

Eukaryotic RNA Polymerase III (Pol III) is a multi-subunit enzyme responsible for transcribing tRNA, 5S rRNA, and several small RNAs. Of the 17 subunits in Pol III, the C17 (Rpc17) and C25 (Rpc25) subunits form a stable subcomplex that protrudes from the core polymerase. In the present study, we determined the crystal structure of the C17/25 subcomplex from Schizosaccharomyces pombe. The subcomplex adopts an elongated shape, and each subunit has two domains. The two subunits in the subcomplex are tightly packed and extensively interact, with a contact area of 2,080 A(2) . The overall conformation of S. pombe C17/25 is considerably different from the previously reported structure of C17/25 from Saccharomyces cerevisiae, with respect to the position of the C17 HRDC domain, a helix-bundle essential for cell viability. In contrast, the S. pombe C17/25 structure is quite similar to those of the Pol II and archaeal counterparts, Rpb4/7 and RpoE/F, respectively, despite the low sequence similarity. A phylogenetic comparison of the C17 subunits among eukaryotes revealed that they can be classified into three groups, according to the length of the inter-domain linker. S. pombe C17, as well as Rpb4 and RpoF, belongs to the largest group, with the short linker. On the other hand, S. cerevisiae C17 belongs to the smallest group, with the long linker, which probably enables the subcomplex to assume the alternative conformation.

Crystal structure of the C17/25 subcomplex from Schizosaccharomyces pombe RNA Polymerase III.,Ehara H, Sekine SI, Yokoyama S Protein Sci. 2011 Jun 28. doi: 10.1002/pro.682. PMID:21714024^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Proshkina GM, Shematorova EK, Proshkin SA, Zaros C, Thuriaux P, Shpakovski GV. Ancient origin, functional conservation and fast evolution of DNA-dependent RNA polymerase III. Nucleic Acids Res. 2006 Jul 28;34(13):3615-24. Print 2006. PMID:16877568 doi:34/13/3615
↑ Proshkina GM, Shematorova EK, Proshkin SA, Zaros C, Thuriaux P, Shpakovski GV. Ancient origin, functional conservation and fast evolution of DNA-dependent RNA polymerase III. Nucleic Acids Res. 2006 Jul 28;34(13):3615-24. Print 2006. PMID:16877568 doi:34/13/3615
↑ Zaros C, Thuriaux P. Rpc25, a conserved RNA polymerase III subunit, is critical for transcription initiation. Mol Microbiol. 2005 Jan;55(1):104-14. PMID:15612920 doi:10.1111/j.1365-2958.2004.04375.x
↑ Ehara H, Sekine SI, Yokoyama S. Crystal structure of the C17/25 subcomplex from Schizosaccharomyces pombe RNA Polymerase III. Protein Sci. 2011 Jun 28. doi: 10.1002/pro.682. PMID:21714024 doi:10.1002/pro.682

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3ayh"

Categories: Fission yeast | Ehara, H | Sekine, S | Yokoyama, S | Transcription

@@ Line 1: / Line 1: @@
 ==Crystal structure of the C17/25 subcomplex from S. pombe RNA Polymerase III==
 <StructureSection load='3ayh' size='340' side='right' caption='[[3ayh]], [[Resolution|resolution]] 2.19&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3ayh]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe_972h- Schizosaccharomyces pombe 972h-]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AYH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3AYH FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3ayh]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Fission_yeast Fission yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AYH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3AYH FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">rpc17, SPAPB1E7.10 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 Schizosaccharomyces pombe 972h-]), rpc25, SPBC2G5.07c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 Schizosaccharomyces pombe 972h-])</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">rpc17, SPAPB1E7.10 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 Fission yeast]), rpc25, SPBC2G5.07c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 Fission yeast])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ayh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ayh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ayh RCSB], [http://www.ebi.ac.uk/pdbsum/3ayh PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ayh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ayh OCA], [http://pdbe.org/3ayh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ayh RCSB], [http://www.ebi.ac.uk/pdbsum/3ayh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ayh ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 17: / Line 18: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 3ayh" style="background-color:#fffaf0;"></div>
 ==See Also==
@@ Line 24: / Line 26: @@
 __TOC__
 </StructureSection>
-[[Category: Schizosaccharomyces pombe 972h-]]
+[[Category: Fission yeast]]
 [[Category: Ehara, H]]
 [[Category: Sekine, S]]
 [[Category: Yokoyama, S]]
 [[Category: Transcription]]

3ayh

From Proteopedia

Revision as of 14:34, 4 August 2016

Crystal structure of the C17/25 subcomplex from S. pombe RNA Polymerase III

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox