2cxc
From Proteopedia
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|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
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| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cxc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cxc OCA], [http://www.ebi.ac.uk/pdbsum/2cxc PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2cxc RCSB]</span> | ||
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[[Category: transcription termination]] | [[Category: transcription termination]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:27:35 2008'' |
Revision as of 23:27, 30 March 2008
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| , resolution 2.00Å | |||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of archaeal transcription termination factor NusA
Overview
The transcription factor NusA functions in transcriptional regulation involving termination in bacteria. A NusA homolog consisting of only the two KH domains is widely conserved in archaea, but its function remains unknown. We have found that Aeropyrum pernix NusA strongly binds to a certain CU-rich sequence near a termination signal. Our crystal structure of A. pernix NusA revealed that its spatial arrangement is quite similar to that of the KH domains of bacterial NusA. Thus, we consider archaeal NusA to have retained some functions of bacterial NusA, including the ssRNA-binding ability. Remarkable structural differences between archaeal and bacterial NusA exist at the interface with RNAP, in connection with the different NusA-binding sites around the termination signals. Transcriptional termination in archaea could differ from all of the known bacterial and eukaryal mechanisms, in terms of the combination of a bacterial factor and a eukaryal-type RNAP.
About this Structure
2CXC is a Single protein structure of sequence from Aeropyrum pernix. Full crystallographic information is available from OCA.
Reference
Crystal structure and RNA-binding analysis of the archaeal transcription factor NusA., Shibata R, Bessho Y, Shinkai A, Nishimoto M, Fusatomi E, Terada T, Shirouzu M, Yokoyama S, Biochem Biophys Res Commun. 2007 Mar 30;355(1):122-8. Epub 2007 Jan 31. PMID:17288993
Page seeded by OCA on Mon Mar 31 02:27:35 2008
Categories: Aeropyrum pernix | Single protein | Bessho, Y. | RSGI, RIKEN Structural Genomics/Proteomics Initiative. | Shibata, R. | Shirouzu, M. | Yokoyama, S. | Archaeal nusa | Kh domain | National project on protein structural and functional analyse | Nppsfa | Riken structural genomics/proteomics initiative | Rna binding protein | Rsgi | Structural genomic | Transcription termination
