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3pxn

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Revision as of 18:01, 4 August 2016

Crystal structure of the Drosophila kinesin family member Kin10/NOD in complex with divalent manganese and ADP

Structural highlights

3pxn is a 1 chain structure with sequence from Drome. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Related:	3dc4, 3dcb, 3dco
Gene:	nod, NODA, CG1763 (DROME)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[NOD_DROME] Required for the distributive chromosome segregation of non-exchange chromosomes during meiosis. May be a microtubule motor required to hold distributively "paired" chromosomes at the metaphase plate until anaphase.

Publication Abstract from PubMed

Kinesins are molecular motors that require a divalent metal ion (for example, Mg(2+)) to convert the energy of ATP hydrolysis into directed force production along microtubules. Here we present the crystal structure of a recombinant kinesin motor domain bound to Mn(2+) and ADP and report on a serine-to-cysteine substitution in the switch 1 motif of kinesin that allows its ATP hydrolysis activity to be controlled by adjusting the ratio of Mn(2+) to Mg(2+). This mutant kinesin binds ATP similarly in the presence of either metal ion, but its ATP hydrolysis activity is greatly diminished in the presence of Mg(2+). In human kinesin-1 and kinesin-5 as well as Drosophila melanogaster kinesin-10 and kinesin-14, this defect is rescued by Mn(2+), providing a way to control both the enzymatic activity and force-generating ability of these nanomachines.

A metal switch for controlling the activity of molecular motor proteins.,Cochran JC, Zhao YC, Wilcox DE, Kull FJ Nat Struct Mol Biol. 2011 Dec 25;19(1):122-7. doi: 10.1038/nsmb.2190. PMID:22198464^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Cochran JC, Zhao YC, Wilcox DE, Kull FJ. A metal switch for controlling the activity of molecular motor proteins. Nat Struct Mol Biol. 2011 Dec 25;19(1):122-7. doi: 10.1038/nsmb.2190. PMID:22198464 doi:http://dx.doi.org/10.1038/nsmb.2190

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3pxn"

@@ Line 1: / Line 1: @@
 ==Crystal structure of the Drosophila kinesin family member Kin10/NOD in complex with divalent manganese and ADP==
 <StructureSection load='3pxn' size='340' side='right' caption='[[3pxn]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
@@ Line 6: / Line 7: @@
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3dc4|3dc4]], [[3dcb|3dcb]], [[3dco|3dco]]</td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">nod, NODA, CG1763 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pxn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pxn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3pxn RCSB], [http://www.ebi.ac.uk/pdbsum/3pxn PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pxn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pxn OCA], [http://pdbe.org/3pxn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3pxn RCSB], [http://www.ebi.ac.uk/pdbsum/3pxn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3pxn ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 18: / Line 19: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 3pxn" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>

3pxn

From Proteopedia

Revision as of 18:01, 4 August 2016

Crystal structure of the Drosophila kinesin family member Kin10/NOD in complex with divalent manganese and ADP

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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