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4co6

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Revision as of 07:16, 23 December 2016

Crystal structure of the Nipah virus RNA free nucleoprotein- phosphoprotein complex

Structural highlights

4co6 is a 6 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
NonStd Res:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[NCAP_NIPAV] Encapsidates the genome protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid (NC) and serves as template for transcription and replication (By similarity). [PHOSP_NIPAV] Essential component of the RNA polymerase transcription and replication complex. Binds the viral ribonucleocapsid and positions the L polymerase on the template (By similarity). May play a role to prevent the establishment of cellular antiviral state by binding to host STAT1 in the cytoplasm. This activity is not as strong as that of V and W.

Publication Abstract from PubMed

Nipah virus (NiV) is a highly pathogenic emergent paramyxovirus causing deadly encephalitis in humans. Its replication requires a constant supply of unassembled nucleoprotein (N0) in complex with its viral chaperone, the phosphoprotein (P). To elucidate the chaperone function of P, we reconstituted NiV the N0-P core complex and determined its crystal structure. The binding of the N-terminal region of P blocks the polymerization of N by interfering with subdomain exchange between N protomers and keeps N0 in an open conformation, ready to grasp an RNA molecule. We found that a peptide derived from the N-binding region of P protects cells against viral infection and demonstrated by structure-based mutagenesis that this peptide acts by inhibiting N0-P formation. These results provide new insights about the assembly of N along genomic RNA and validate the N0-P complex as a target for drug development.

Structure of Nipah virus unassembled nucleoprotein in complex with its viral chaperone.,Yabukarski F, Lawrence P, Tarbouriech N, Bourhis JM, Delaforge E, Jensen MR, Ruigrok RW, Blackledge M, Volchkov V, Jamin M Nat Struct Mol Biol. 2014 Aug 10. doi: 10.1038/nsmb.2868. PMID:25108352^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Yabukarski F, Lawrence P, Tarbouriech N, Bourhis JM, Delaforge E, Jensen MR, Ruigrok RW, Blackledge M, Volchkov V, Jamin M. Structure of Nipah virus unassembled nucleoprotein in complex with its viral chaperone. Nat Struct Mol Biol. 2014 Aug 10. doi: 10.1038/nsmb.2868. PMID:25108352 doi:http://dx.doi.org/10.1038/nsmb.2868

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4co6"

@@ Line 1: / Line 1: @@
 ==Crystal structure of the Nipah virus RNA free nucleoprotein- phosphoprotein complex==
 <StructureSection load='4co6' size='340' side='right' caption='[[4co6]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
 <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4co6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4co6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4co6 RCSB], [http://www.ebi.ac.uk/pdbsum/4co6 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4co6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4co6 OCA], [http://pdbe.org/4co6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4co6 RCSB], [http://www.ebi.ac.uk/pdbsum/4co6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4co6 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 17: / Line 18: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4co6" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Nucleoprotein|Nucleoprotein]]
 == References ==
 <references/>

4co6

From Proteopedia

Revision as of 07:16, 23 December 2016

Crystal structure of the Nipah virus RNA free nucleoprotein- phosphoprotein complex

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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