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4lvh

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Revision as of 18:49, 4 August 2016

Insight into highly conserved H1 subtype-specific epitopes in influenza virus hemagglutinin

Structural highlights

4lvh is a 12 chain structure with sequence from Influenza a virus (a/korea/01/2009(h1n1)) and Mus musculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	4eda, 4f15
Gene:	HA (Influenza A virus (A/Korea/01/2009(H1N1)))
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[C5MQE6_9INFA] Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore (By similarity).[RuleBase:RU003324][SAAS:SAAS008980_004_327643]

Publication Abstract from PubMed

Influenza viruses continuously undergo antigenic changes with gradual accumulation of mutations in hemagglutinin (HA) that is a major determinant in subtype specificity. The identification of conserved epitopes within specific HA subtypes gives an important clue for developing new vaccines and diagnostics. We produced and characterized nine monoclonal antibodies that showed significant neutralizing activities against H1 subtype influenza viruses, and determined the complex structure of HA derived from a 2009 pandemic virus A/Korea/01/2009 (KR01) and the Fab fragment from H1-specific monoclonal antibody GC0587. The overall structure of the complex was essentially identical to the previously determined KR01 HA-Fab0757 complex structure. Both Fab0587 and Fab0757 recognize readily accessible head regions of HA, revealing broadly shared and conserved antigenic determinants among H1 subtypes. The beta-strands constituted by Ser110-Glu115 and Lys169-Lys170 form H1 epitopes with distinct conformations from those of H1 and H3 HA sites. In particular, Glu112, Glu115, Lys169, and Lys171 that are highly conserved among H1 subtype HAs have close contacts with HCDR3 and LCDR3. The differences between Fab0587 and Fab0757 complexes reside mainly in HCDR3 and LCDR3, providing distinct antigenic determinants specific for 1918 pdm influenza strain. Our results demonstrate a potential key neutralizing epitope important for H1 subtype specificity in influenza virus.

Insight into highly conserved h1 subtype-specific epitopes in influenza virus hemagglutinin.,Cho KJ, Hong KW, Kim SH, Seok JH, Kim S, Lee JH, Saelens X, Kim KH PLoS One. 2014 Feb 26;9(2):e89803. doi: 10.1371/journal.pone.0089803. eCollection, 2014. PMID:24587046^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Cho KJ, Hong KW, Kim SH, Seok JH, Kim S, Lee JH, Saelens X, Kim KH. Insight into highly conserved h1 subtype-specific epitopes in influenza virus hemagglutinin. PLoS One. 2014 Feb 26;9(2):e89803. doi: 10.1371/journal.pone.0089803. eCollection, 2014. PMID:24587046 doi:http://dx.doi.org/10.1371/journal.pone.0089803

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4lvh"

@@ Line 1: / Line 1: @@
 ==Insight into highly conserved H1 subtype-specific epitopes in influenza virus hemagglutinin==
 <StructureSection load='4lvh' size='340' side='right' caption='[[4lvh]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
@@ Line 6: / Line 7: @@
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4eda|4eda]], [[4f15|4f15]]</td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">HA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=644289 Influenza A virus (A/Korea/01/2009(H1N1))])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4lvh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lvh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4lvh RCSB], [http://www.ebi.ac.uk/pdbsum/4lvh PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4lvh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lvh OCA], [http://pdbe.org/4lvh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4lvh RCSB], [http://www.ebi.ac.uk/pdbsum/4lvh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4lvh ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 18: / Line 19: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4lvh" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>

4lvh

From Proteopedia

Revision as of 18:49, 4 August 2016

Insight into highly conserved H1 subtype-specific epitopes in influenza virus hemagglutinin

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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