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3t72

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Revision as of 20:42, 4 August 2016

PhoB(E)-Sigma70(4)-(RNAP-Betha-flap-tip-helix)-DNA Transcription Activation Sub-Complex

Structural highlights

3t72 is a 50 chain structure with sequence from Ecoli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	phoB, b0399, JW0389 (ECOLI), rpoD, alt, b3067, JW3039, EKO11_4334, rpoB (ECOLI)
Activity:	DNA-directed RNA polymerase, with EC number 2.7.7.6
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[RPOD_ECOLI] Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium. [PHOB_ECOLI] This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by PhoB and PhoR when phosphate is limited.

Publication Abstract from PubMed

PhoB is a two-component response regulator that activates transcription by interacting with the sigma(70) subunit of the E. coli RNA polymerase in promoters in which the -35 sigma(70)-recognition element is replaced by the pho box. The crystal structure of a transcription initiation subcomplex that includes the sigma(4) domain of sigma(70) fused with the RNA polymerase beta subunit flap tip helix, the PhoB effector domain and the pho box DNA reveals how sigma(4) recognizes the upstream pho box repeat. As with the -35 element, sigma(4) achieves this recognition through the N-terminal portion of its DNA recognition helix, but contact with the DNA major groove is less extensive. Unexpectedly, the same recognition helix contacts the transactivation loop and helices alpha2 and alpha3 of PhoB. This result shows a simple and elegant mechanism for polymerase recruitment to pho box promoters in which the lost -35 element contacts are compensated by new ones with the activator. In addition, sigma(4) is reoriented, thereby suggesting a remodelling mechanism for transcription initiation.

The structure of a transcription activation subcomplex reveals how sigma(70) is recruited to PhoB promoters.,Blanco AG, Canals A, Bernues J, Sola M, Coll M EMBO J. 2011 Aug 9;30(18):3776-85. doi: 10.1038/emboj.2011.271. PMID:21829166^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Blanco AG, Canals A, Bernues J, Sola M, Coll M. The structure of a transcription activation subcomplex reveals how sigma(70) is recruited to PhoB promoters. EMBO J. 2011 Aug 9;30(18):3776-85. doi: 10.1038/emboj.2011.271. PMID:21829166 doi:10.1038/emboj.2011.271

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3t72"

@@ Line 1: / Line 1: @@
 ==PhoB(E)-Sigma70(4)-(RNAP-Betha-flap-tip-helix)-DNA Transcription Activation Sub-Complex==
 <StructureSection load='3t72' size='340' side='right' caption='[[3t72]], [[Resolution|resolution]] 4.33&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">phoB, b0399, JW0389 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI]), rpoD, alt, b3067, JW3039, EKO11_4334, rpoB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t72 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t72 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3t72 RCSB], [http://www.ebi.ac.uk/pdbsum/3t72 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t72 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t72 OCA], [http://pdbe.org/3t72 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3t72 RCSB], [http://www.ebi.ac.uk/pdbsum/3t72 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3t72 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 17: / Line 18: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 3t72" style="background-color:#fffaf0;"></div>
 ==See Also==

3t72

From Proteopedia

Revision as of 20:42, 4 August 2016

PhoB(E)-Sigma70(4)-(RNAP-Betha-flap-tip-helix)-DNA Transcription Activation Sub-Complex

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

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