2dpe
From Proteopedia
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|PDB= 2dpe |SIZE=350|CAPTION= <scene name='initialview01'>2dpe</scene>, resolution 2.07Å | |PDB= 2dpe |SIZE=350|CAPTION= <scene name='initialview01'>2dpe</scene>, resolution 2.07Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= | + | |LIGAND= <scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1ljy|1LJY]], [[2esc|2ESC]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2dpe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2dpe OCA], [http://www.ebi.ac.uk/pdbsum/2dpe PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2dpe RCSB]</span> | ||
}} | }} | ||
| Line 34: | Line 37: | ||
[[Category: signaling protein]] | [[Category: signaling protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:37:38 2008'' |
Revision as of 23:37, 30 March 2008
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| , resolution 2.07Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , | ||||||
| Related: | 1LJY, 2ESC
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of a secretory 40KDA glycoprotein from sheep at 2.0A resolution
Overview
A 40kDa glycoprotein from dry secretion of sheep is implicated as a signaling factor and is named as SPS-40. This protein is secreted only during the early phase of involution when the drastic tissue remodeling occurs in the mammary gland. SPS-40 was purified from sheep dry secretions and crystallized using hanging drop vapour diffusion method. The crystals belong to orthorhombic space group P2(1)2(1)2(1) with cell dimensions, a=62.7A, b=66.4A, c=107.5A. The protein was also cloned for the determination of its complete amino acid sequence. The three-dimensional structure of SPS-40 was determined by X-ray crystallographic method at 2.0A resolution. The structure revealed the presence of an N-linked glycan chain at Asn39. The protein adopts a conformation with a classical (beta/alpha)(8)-barrel fold of triosephosphate isomerase (TIM) (residues 1-237 and 310-360) with an insertion of a small (alpha+beta) domain (residues 240-307) similar to that observed in chitinases. However, the Leu substitution for Glu in the consensus catalytic sequence in SPS-40 causes a loss of chitinase activity. Furthermore, the sugar-binding groove in SPS-40 is distorted considerably from the standard chitin-binding site in chitinase enzymes and hence the binding of chitin-like oligosaccharides is considerably hampered. Three surface loops, His188-His197, Phe202-Arg212 and Phe244-Pro260 have exceptionally high values of B-factors (average=70.5A(2)), indicating the presence of a less defined region.
About this Structure
2DPE is a Single protein structure of sequence from Ovis aries. This structure supersedes the now removed PDB entry 1R2V. Full crystallographic information is available from OCA.
Reference
Crystal structure of a secretory signalling glycoprotein from sheep at 2.0A resolution., Srivastava DB, Ethayathulla AS, Kumar J, Singh N, Sharma S, Das U, Srinivasan A, Singh TP, J Struct Biol. 2006 Dec;156(3):505-16. Epub 2006 Jun 8. PMID:16859926
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