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2iz9

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Revision as of 08:43, 11 September 2015

MS2-RNA HAIRPIN (4ONE-5) COMPLEX

Structural highlights

2iz9 is a 5 chain structure with sequence from Bacteriophage ms2. This structure supersedes the now removed PDB entry 1dzs. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
NonStd Res:
Related:	1aq3, 1aq4, 1bms, 1msc, 1mst, 1mva, 1mvb, 1u1y, 1zdh, 1zdi, 1zdj, 1zdk, 1zse, 2b2d, 2b2e, 2b2g, 2bny, 2bq5, 2bs0, 2bs1, 2bu1, 2c4q, 2c4y, 2c4z, 2c50, 2c51, 2ms2, 5msf, 6msf, 7msf, 2iz8, 2izm, 2izn
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum

Function

[COAT_BPMS2] Forms the phage shell; binds to the phage RNA.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The MS2 coat protein binds specifically to an RNA hairpin formed within the viral genome. By soaking different RNA fragments into crystals of MS2 coat protein capsids it is possible to determine the X-ray structure of the RNA-protein complexes formed. Here we present the structure to 2.85 A resolution of a complex between a chemically modified RNA hairpin variant and the MS2 coat protein. This RNA variant has a substitution at the -5 base position, which has been shown previously to be pyrimidine-specific and is a uracil in the wild-type RNA. The modified RNA hairpin contains a pyridin-4-one base (4one) at this position that lacks the exocyclic 2-oxygen eliminating the possibility of forming a hydrogen bond to asparagine A87 in the protein. The 4one complex structure shows an unprecedented major conformational change in the loop region of the RNA, whereas there is almost no change in the conformation of the protein.

Deletion of a single hydrogen bonding atom from the MS2 RNA operator leads to dramatic rearrangements at the RNA-coat protein interface.,Grahn E, Stonehouse NJ, Adams CJ, Fridborg K, Beigelman L, Matulic-Adamic J, Warriner SL, Stockley PG, Liljas L Nucleic Acids Res. 2000 Dec 1;28(23):4611-6. PMID:11095669^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Grahn E, Stonehouse NJ, Adams CJ, Fridborg K, Beigelman L, Matulic-Adamic J, Warriner SL, Stockley PG, Liljas L. Deletion of a single hydrogen bonding atom from the MS2 RNA operator leads to dramatic rearrangements at the RNA-coat protein interface. Nucleic Acids Res. 2000 Dec 1;28(23):4611-6. PMID:11095669

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2iz9"

@@ Line 2: / Line 2: @@
 <StructureSection load='2iz9' size='340' side='right' caption='[[2iz9]], [[Resolution|resolution]] 2.85&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2iz9]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacterio_phage_ms2 Enterobacterio phage ms2]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1dzs 1dzs]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IZ9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2IZ9 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2iz9]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacteriophage_ms2 Bacteriophage ms2]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1dzs 1dzs]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IZ9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2IZ9 FirstGlance]. <br>
 </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ONE:1-(BETA-D-RIBOFURANOSYL)-PYRIDIN-4-ONE-5-PHOSPHATE'>ONE</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1aq3|1aq3]], [[1aq4|1aq4]], [[1bms|1bms]], [[1msc|1msc]], [[1mst|1mst]], [[1mva|1mva]], [[1mvb|1mvb]], [[1u1y|1u1y]], [[1zdh|1zdh]], [[1zdi|1zdi]], [[1zdj|1zdj]], [[1zdk|1zdk]], [[1zse|1zse]], [[2b2d|2b2d]], [[2b2e|2b2e]], [[2b2g|2b2g]], [[2bny|2bny]], [[2bq5|2bq5]], [[2bs0|2bs0]], [[2bs1|2bs1]], [[2bu1|2bu1]], [[2c4q|2c4q]], [[2c4y|2c4y]], [[2c4z|2c4z]], [[2c50|2c50]], [[2c51|2c51]], [[2ms2|2ms2]], [[5msf|5msf]], [[6msf|6msf]], [[7msf|7msf]], [[2iz8|2iz8]], [[2izm|2izm]], [[2izn|2izn]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2iz9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iz9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2iz9 RCSB], [http://www.ebi.ac.uk/pdbsum/2iz9 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2iz9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iz9 OCA], [http://pdbe.org/2iz9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2iz9 RCSB], [http://www.ebi.ac.uk/pdbsum/2iz9 PDBsum]</span></td></tr>
 </table>
 == Function ==
@@ Line 27: / Line 27: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 2iz9" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Enterobacterio phage ms2]]
+[[Category: Bacteriophage ms2]]
 [[Category: Adams, C J]]
 [[Category: Beigelman, L]]

2iz9

From Proteopedia

Revision as of 08:43, 11 September 2015

MS2-RNA HAIRPIN (4ONE-5) COMPLEX

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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