2dry
From Proteopedia
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|PDB= 2dry |SIZE=350|CAPTION= <scene name='initialview01'>2dry</scene>, resolution 1.80Å | |PDB= 2dry |SIZE=350|CAPTION= <scene name='initialview01'>2dry</scene>, resolution 1.80Å | ||
|SITE= <scene name='pdbsite=AC1:So4+Binding+Site+For+Residue+A+2001'>AC1</scene>, <scene name='pdbsite=AC2:So4+Binding+Site+For+Residue+A+2002'>AC2</scene>, <scene name='pdbsite=AC3:So4+Binding+Site+For+Residue+A+2003'>AC3</scene>, <scene name='pdbsite=AC4:So4+Binding+Site+For+Residue+A+2004'>AC4</scene>, <scene name='pdbsite=AC5:Pge+Binding+Site+For+Residue+B+1001'>AC5</scene> and <scene name='pdbsite=AC6:Pge+Binding+Site+For+Residue+A+1002'>AC6</scene> | |SITE= <scene name='pdbsite=AC1:So4+Binding+Site+For+Residue+A+2001'>AC1</scene>, <scene name='pdbsite=AC2:So4+Binding+Site+For+Residue+A+2002'>AC2</scene>, <scene name='pdbsite=AC3:So4+Binding+Site+For+Residue+A+2003'>AC3</scene>, <scene name='pdbsite=AC4:So4+Binding+Site+For+Residue+A+2004'>AC4</scene>, <scene name='pdbsite=AC5:Pge+Binding+Site+For+Residue+B+1001'>AC5</scene> and <scene name='pdbsite=AC6:Pge+Binding+Site+For+Residue+A+1002'>AC6</scene> | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[2ao3|2AO3]], [[2d12|2D12]], [[2drz|2DRZ]], [[2ds0|2DS0]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2dry FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2dry OCA], [http://www.ebi.ac.uk/pdbsum/2dry PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2dry RCSB]</span> | ||
}} | }} | ||
| Line 24: | Line 27: | ||
[[Category: Fujimoto, Z.]] | [[Category: Fujimoto, Z.]] | ||
[[Category: Suzuki, R.]] | [[Category: Suzuki, R.]] | ||
| - | [[Category: PGE]] | ||
| - | [[Category: SO4]] | ||
[[Category: beta-trefoil fold]] | [[Category: beta-trefoil fold]] | ||
[[Category: earthworm lumbricus terrestri]] | [[Category: earthworm lumbricus terrestri]] | ||
| Line 33: | Line 34: | ||
[[Category: sugar binding protein]] | [[Category: sugar binding protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:38:43 2008'' |
Revision as of 23:38, 30 March 2008
| |||||||
| , resolution 1.80Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | , , , , and | ||||||
| Ligands: | , | ||||||
| Related: | 2AO3, 2D12, 2DRZ, 2DS0
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of the earthworm lectin C-terminal domain mutant
Overview
Sialic acid (Sia) is a typical terminal sugar, which modifies various types of glycoconjugates commonly found in higher animals. Its regulatory roles in diverse biological phenomena are frequently triggered by interaction with Sia-binding lectins. When using natural Sia-binding lectins as probes, however, there have been practical problems concerning their repertoire and availability. Here, we show a rational creation of a Sia-binding lectin based on the strategy 'natural evolution-mimicry', where Sia-binding lectins are engineered by error-prone PCR from a Gal-binding lectin used as a scaffold protein. After selection with fetuin-agarose using a recently reinforced ribosome display system, one of the evolved mutants SRC showed substantial affinity for alpha2-6Sia, which the parental Gal-binding lectin EW29Ch lacked. SRC was found to have additional practical advantages in productivity and in preservation of affinity for Gal. Thus, the developed novel Sia-recognition protein will contribute as useful tools to sialoglycomics.
About this Structure
2DRY is a Single protein structure of sequence from Lumbricus terrestris. Full crystallographic information is available from OCA.
Reference
Tailoring a novel sialic acid-binding lectin from a ricin-B chain-like galactose-binding protein by natural evolution-mimicry., Yabe R, Suzuki R, Kuno A, Fujimoto Z, Jigami Y, Hirabayashi J, J Biochem. 2007 Mar;141(3):389-99. Epub 2007 Jan 18. PMID:17234683
Page seeded by OCA on Mon Mar 31 02:38:43 2008
