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1hqz

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Revision as of 10:49, 11 September 2015

Cofilin homology domain of a yeast actin-binding protein ABP1P

Structural highlights

1hqz is a 9 chain structure with sequence from Atcc 18824. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, TOPSAN

Function

[ABP1_YEAST] Regulates ARP2/3 complex-mediated actin assembly. Recruits ARP2/3 complex to sides of preexisting actin filaments, which may promote nucleation or stabilization of filament branches. Binds to actin filaments, but not actin monomers. Actin binding is required for ARP2/3 complex activation. May also have a role in linking the actin cytoskeleton to endocytosis. recruits components of the endocytotic machinery to cortical actin patches, known sites of endocytosis.^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

A modified molecular-replacement method is described that makes use of six-dimensional searches and the phased translation function, providing a systematic examination of all possible search-model orientations in an experimental electron-density map. As an example, the structure solution of the cofilin-homology domain of the Saccharomyces cerevisiae actin-binding protein 1 (ABP1) is presented in detail. Additional examples are presented in which these tools have significantly aided structure solutions in a variety of contexts. These results suggest that this approach might be of widespread utility for challenging structures involving weak phase information, complex asymmetric units and search models with weak structural homology. Furthermore, this approach supports an exhaustive molecular-replacement strategy in cases where an appropriate search model cannot readily be identified on the basis of sequence homology. The fully automated web-based implementation of this phased translation function is described.

Phased translation function revisited: structure solution of the cofilin-homology domain from yeast actin-binding protein 1 using six-dimensional searches.,Strokopytov BV, Fedorov A, Mahoney NM, Kessels M, Drubin DG, Almo SC Acta Crystallogr D Biol Crystallogr. 2005 Mar;61(Pt 3):285-93. Epub 2005, Feb 24. PMID:15735338^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Goode BL, Rodal AA, Barnes G, Drubin DG. Activation of the Arp2/3 complex by the actin filament binding protein Abp1p. J Cell Biol. 2001 Apr 30;153(3):627-34. PMID:11331312
↑ Strokopytov BV, Fedorov A, Mahoney NM, Kessels M, Drubin DG, Almo SC. Phased translation function revisited: structure solution of the cofilin-homology domain from yeast actin-binding protein 1 using six-dimensional searches. Acta Crystallogr D Biol Crystallogr. 2005 Mar;61(Pt 3):285-93. Epub 2005, Feb 24. PMID:15735338 doi:10.1107/S0907444904033037

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1hqz"

@@ Line 2: / Line 2: @@
 <StructureSection load='1hqz' size='340' side='right' caption='[[1hqz]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1hqz]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HQZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1HQZ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1hqz]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HQZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1HQZ FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hqz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hqz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1hqz RCSB], [http://www.ebi.ac.uk/pdbsum/1hqz PDBsum], [http://www.topsan.org/Proteins/NYSGXRC/1hqz TOPSAN]</span></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hqz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hqz OCA], [http://pdbe.org/1hqz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1hqz RCSB], [http://www.ebi.ac.uk/pdbsum/1hqz PDBsum], [http://www.topsan.org/Proteins/NYSGXRC/1hqz TOPSAN]</span></td></tr>
 </table>
 == Function ==
@@ Line 25: / Line 25: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 1hqz" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Saccharomyces cerevisiae]]
+[[Category: Atcc 18824]]
 [[Category: Almo, S C]]
 [[Category: Burley, S K]]

1hqz

From Proteopedia

Revision as of 10:49, 11 September 2015

Cofilin homology domain of a yeast actin-binding protein ABP1P

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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