2e31

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|ACTIVITY=
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|RELATEDENTRY=[[2e32|2E32]], [[2e33|2E33]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2e31 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2e31 OCA], [http://www.ebi.ac.uk/pdbsum/2e31 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2e31 RCSB]</span>
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[[Category: ubiquitin ligase]]
[[Category: ubiquitin ligase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:34:38 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:43:00 2008''

Revision as of 23:43, 30 March 2008

Image:2e31.jpg


PDB ID 2e31

Drag the structure with the mouse to rotate
, resolution 2.40Å
Related: 2E32, 2E33


Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



Structural basis for selection of glycosylated substrate by SCFFbs1 ubiquitin ligase


Overview

The ubiquitin ligase complex SCF(Fbs1), which contributes to the ubiquitination of glycoproteins, is involved in the endoplasmic reticulum-associated degradation pathway. In SCF ubiquitin ligases, a diverse array of F-box proteins confers substrate specificity. Fbs1/Fbx2, a member of the F-box protein family, recognizes high-mannose oligosaccharides. To elucidate the structural basis of SCF(Fbs1) function, we determined the crystal structures of the Skp1-Fbs1 complex and the sugar-binding domain (SBD) of the Fbs1-glycoprotein complex. The mechanistic model indicated by the structures appears to be well conserved among the SCF ubiquitin ligases. The structure of the SBD-glycoprotein complex indicates that the SBD primarily recognizes Man(3)GlcNAc(2), thereby explaining the broad activity of the enzyme against various glycoproteins. Comparison of two crystal structures of the Skp1-Fbs1 complex revealed the relative motion of a linker segment between the F-box and the SBD domains, which might underlie the ability of the complex to recognize different acceptor lysine residues for ubiquitination.

About this Structure

2E31 is a Protein complex structure of sequences from Homo sapiens and Mus musculus. Full crystallographic information is available from OCA.

Reference

Structural basis for the selection of glycosylated substrates by SCF(Fbs1) ubiquitin ligase., Mizushima T, Yoshida Y, Kumanomidou T, Hasegawa Y, Suzuki A, Yamane T, Tanaka K, Proc Natl Acad Sci U S A. 2007 Apr 3;104(14):5777-81. Epub 2007 Mar 26. PMID:17389369

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