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3at5

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Revision as of 07:11, 5 August 2016

Side-necked turtle (Pleurodira, Chelonia, REPTILIA) hemoglobin: cDNA-derived primary structures and X-ray crystal structures of Hb A

Structural highlights

3at5 is a 2 chain structure with sequence from Podocnemis unifilis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	3at6
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

Red blood cells of yellow-spotted river turtles (Podocnemis unifilis, Pleurodira, Chelonia, REPTILIA) have two hemoglobin (Hb) components, Hb A and Hb D. We purified the hemoglobin component homologous to amniote (reptiles, birds, and mammals) adult Hb A which comprises two identical alpha(A) -globin polypeptides and two identical beta-globin polypeptides. To establish the crystal structure of Podocnemis Hb A, we first determined the globin primary structures using cDNA nucleotide sequencing with the assistance of protein sequencing. The purified Podocnemis Hb A produced a different form of crystal for each of the two different buffer systems used: form A, tetragonal crystals (space group, P4(1) 2(1) 2), produced under neutral pH (pH 7-8) conditions; and form B, hexagonal crystals (space group, P6(1) 22), produced under high alkaline pH (pH 11-13) conditions. Single crystals of the two forms were examined by Raman microscopy with an excitation of 532 nm, indicating their structural differences. The crystal structures of the two forms were constructed by X-ray crystallographic diffraction at a resolution of 2.20 A for form A and 2.35 A for form B. The differences of the tertiary and quaternary structures of the two forms were marginal; however, one clear difference was found in helix structure. When comparing Podocnemis Hb A with Hb A from specimens in other taxa, such as Anser indicus (birds) and Homo sapiens (mammals) by SHELXPRO, the root mean square deviation (RMSD) between the corresponding Calpha atoms of the two globins does not exceed 2.0 A. These low values indicate the crystal structures resemble each other. Our data on X-ray crystal structures and Raman spectra not only reveal the first findings on the two crystal forms of Podocnemis unifilis Hb A but also provide the first refined models for reptilian adult Hb A. (c) 2011 IUBMB IUBMB Life, 63(3): 188-196, 2011.

Side-necked turtle (Pleurodira, Chelonia, reptilia) hemoglobin: cDNA-derived primary structures and X-ray crystal structures of Hb A.,Hasegawa T, Shishikura F, Kuwada T IUBMB Life. 2011 Mar;63(3):188-96. doi: 10.1002/iub.429. PMID:21445850^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Hasegawa T, Shishikura F, Kuwada T. Side-necked turtle (Pleurodira, Chelonia, reptilia) hemoglobin: cDNA-derived primary structures and X-ray crystal structures of Hb A. IUBMB Life. 2011 Mar;63(3):188-96. doi: 10.1002/iub.429. PMID:21445850 doi:10.1002/iub.429

1 Structural highlights
2 Publication Abstract from PubMed
3 See Also
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3at5"

@@ Line 1: / Line 1: @@
 ==Side-necked turtle (Pleurodira, Chelonia, REPTILIA) hemoglobin: cDNA-derived primary structures and X-ray crystal structures of Hb A==
 <StructureSection load='3at5' size='340' side='right' caption='[[3at5]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3at6|3at6]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3at5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3at5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3at5 RCSB], [http://www.ebi.ac.uk/pdbsum/3at5 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3at5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3at5 OCA], [http://pdbe.org/3at5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3at5 RCSB], [http://www.ebi.ac.uk/pdbsum/3at5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3at5 ProSAT]</span></td></tr>
 </table>
 <div style="background-color:#fffaf0;">
@@ Line 15: / Line 16: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 3at5" style="background-color:#fffaf0;"></div>
 ==See Also==

3at5

From Proteopedia

Revision as of 07:11, 5 August 2016

Side-necked turtle (Pleurodira, Chelonia, REPTILIA) hemoglobin: cDNA-derived primary structures and X-ray crystal structures of Hb A

Structural highlights

Publication Abstract from PubMed

See Also

References

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