3f1j

From Proteopedia

(Difference between revisions)

Revision as of 08:00, 5 August 2016

Crystal structure of the Borna disease virus matrix protein (BDV-M) reveals RNA binding properties

Structural highlights

3f1j is a 1 chain structure with sequence from Bdv. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
NonStd Res:
Gene:	M (BDV)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[MATRX_BDV] Plays a crucial role in virion assembly and budding (By similarity).

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Borna disease virus (BDV) is a neurotropic enveloped RNA virus that causes a noncytolytic, persistent infection of the central nervous system in mammals. BDV belongs to the order Mononegavirales, which also includes the negative-strand RNA viruses (NSVs) Ebola, Marburg, vesicular stomatitis, rabies, mumps, and measles. BDV-M, the matrix protein (M-protein) of BDV, is the smallest M-protein (16.2 kDa) among the NSVs. M-proteins play a critical role in virus assembly and budding, mediating the interaction between the viral capsid, envelope, and glycoprotein spikes, and are as such responsible for the structural stability and individual form of virus particles. Here, we report the 3D structure of BDV-M, a full-length M-protein structure from a nonsegmented RNA NSV. The BDV-M monomer exhibits structural similarity to the N-terminal domain of the Ebola M-protein (VP40), while the surface charge of the tetramer provides clues to the membrane association of BDV-M. Additional electron density in the crystal reveals the presence of bound nucleic acid, interpreted as cytidine-5'-monophosphate. The heterologously expressed BDV-M copurifies with and protects ssRNA oligonucleotides of a median length of 16 nt taken up from the expression host. The results presented here show that BDV-M would be able to bind RNA and lipid membranes simultaneously, expanding the repertoire of M-protein functionalities.

Crystal structure of the Borna disease virus matrix protein (BDV-M) reveals ssRNA binding properties.,Neumann P, Lieber D, Meyer S, Dautel P, Kerth A, Kraus I, Garten W, Stubbs MT Proc Natl Acad Sci U S A. 2009 Mar 10;106(10):3710-5. Epub 2009 Feb 23. PMID:19237566^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Neumann P, Lieber D, Meyer S, Dautel P, Kerth A, Kraus I, Garten W, Stubbs MT. Crystal structure of the Borna disease virus matrix protein (BDV-M) reveals ssRNA binding properties. Proc Natl Acad Sci U S A. 2009 Mar 10;106(10):3710-5. Epub 2009 Feb 23. PMID:19237566

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3f1j"

@@ Line 1: / Line 1: @@
 ==Crystal structure of the Borna disease virus matrix protein (BDV-M) reveals RNA binding properties==
 <StructureSection load='3f1j' size='340' side='right' caption='[[3f1j]], [[Resolution|resolution]] 2.65&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3f1j]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Borna_disease_virus Borna disease virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3F1J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3F1J FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3f1j]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bdv Bdv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3F1J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3F1J FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=C5P:CYTIDINE-5-MONOPHOSPHATE'>C5P</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
 <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">M ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=12455 Borna disease virus])</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">M ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=12455 BDV])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3f1j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3f1j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3f1j RCSB], [http://www.ebi.ac.uk/pdbsum/3f1j PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3f1j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3f1j OCA], [http://pdbe.org/3f1j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3f1j RCSB], [http://www.ebi.ac.uk/pdbsum/3f1j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3f1j ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 18: / Line 19: @@
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
-</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3f1j ConSurf].
 <div style="clear:both"></div>
 <div style="background-color:#fffaf0;">
@@ Line 28: / Line 29: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 3f1j" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Borna disease virus]]
+[[Category: Bdv]]
 [[Category: Dautel, P]]
 [[Category: Garten, W]]

3f1j

From Proteopedia

Revision as of 08:00, 5 August 2016

Crystal structure of the Borna disease virus matrix protein (BDV-M) reveals RNA binding properties

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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