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3tw4

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Revision as of 11:40, 5 August 2016

Crystal Structure of Human Septin 7 GTPase Domain

Structural highlights

3tw4 is a 2 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Gene:	SEPT7, CDC10 (HUMAN)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[SEPT7_HUMAN] Filament-forming cytoskeletal GTPase. Required for normal organization of the actin cytoskeleton. Required for normal progress through mitosis. Involved in cytokinesis. Required for normal association of CENPE with the kinetochore. Plays a role in ciliogenesis and collective cell movements.^[1] ^[2]

Publication Abstract from PubMed

We describe the purification, crystallization and structure for the GTP-binding domain of human septin 7 (SEPT7G). We show that it forms filaments within the crystal lattice which employ both the G and NC interfaces, similar to those seen in the hetero-filament of SEPT2/6/7. The NC interface is considered promiscuous as it is absent from the hetero-filament. Such promiscuity could provide the potential for permuting monomers along a filament in order to generate diversity in hetero-polymers. On the other hand, our results suggest that the G and NC interfaces may be necessary but insufficient for determining correct hetero-filament assembly. STRUCTURED SUMMARY OF PROTEIN INTERACTIONS: SEPT7G and SEPT7Gbind by X-ray crystallography (View interaction). SEPT7 G and SEPT7 Gbind by molecular sieving (View interaction).

Promiscuous interactions of human septins: The GTP binding domain of SEPT7 forms filaments within the crystal.,Serrao VH, Alessandro F, Caldas VE, Marcal RL, D'Muniz Pereira H, Thiemann OH, Garratt RC FEBS Lett. 2011 Nov 3. PMID:22064074^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Kremer BE, Adang LA, Macara IG. Septins regulate actin organization and cell-cycle arrest through nuclear accumulation of NCK mediated by SOCS7. Cell. 2007 Sep 7;130(5):837-50. PMID:17803907 doi:http://dx.doi.org/10.1016/j.cell.2007.06.053
↑ Zhu M, Wang F, Yan F, Yao PY, Du J, Gao X, Wang X, Wu Q, Ward T, Li J, Kioko S, Hu R, Xie W, Ding X, Yao X. Septin 7 interacts with centromere-associated protein E and is required for its kinetochore localization. J Biol Chem. 2008 Jul 4;283(27):18916-25. doi: 10.1074/jbc.M710591200. Epub 2008 , May 6. PMID:18460473 doi:http://dx.doi.org/10.1074/jbc.M710591200
↑ Serrao VH, Alessandro F, Caldas VE, Marcal RL, D'Muniz Pereira H, Thiemann OH, Garratt RC. Promiscuous interactions of human septins: The GTP binding domain of SEPT7 forms filaments within the crystal. FEBS Lett. 2011 Nov 3. PMID:22064074 doi:10.1016/j.febslet.2011.10.043

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3tw4"

@@ Line 1: / Line 1: @@
 ==Crystal Structure of Human Septin 7 GTPase Domain==
 <StructureSection load='3tw4' size='340' side='right' caption='[[3tw4]], [[Resolution|resolution]] 3.35&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3tw4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TW4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TW4 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3tw4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TW4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TW4 FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SEPT7, CDC10 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SEPT7, CDC10 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tw4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tw4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tw4 RCSB], [http://www.ebi.ac.uk/pdbsum/3tw4 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tw4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tw4 OCA], [http://pdbe.org/3tw4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3tw4 RCSB], [http://www.ebi.ac.uk/pdbsum/3tw4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3tw4 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 17: / Line 18: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 3tw4" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Homo sapiens]]
+[[Category: Human]]
 [[Category: Alessandro, F]]
 [[Category: Garratt, R C]]

3tw4

From Proteopedia

Revision as of 11:40, 5 August 2016

Crystal Structure of Human Septin 7 GTPase Domain

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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