3vjp
From Proteopedia
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==Orthorhombic Crystal Structure of Salmonella FlgA in closed form== | ==Orthorhombic Crystal Structure of Salmonella FlgA in closed form== | ||
<StructureSection load='3vjp' size='340' side='right' caption='[[3vjp]], [[Resolution|resolution]] 2.70Å' scene=''> | <StructureSection load='3vjp' size='340' side='right' caption='[[3vjp]], [[Resolution|resolution]] 2.70Å' scene=''> | ||
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3tee|3tee]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3tee|3tee]]</td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">flgA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=90371 "Bacillus typhimurium" Loeffler 1892])</td></tr> | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">flgA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=90371 "Bacillus typhimurium" Loeffler 1892])</td></tr> | ||
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vjp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vjp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vjp RCSB], [http://www.ebi.ac.uk/pdbsum/3vjp PDBsum]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vjp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vjp OCA], [http://pdbe.org/3vjp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3vjp RCSB], [http://www.ebi.ac.uk/pdbsum/3vjp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3vjp ProSAT]</span></td></tr> |
</table> | </table> | ||
== Function == | == Function == | ||
[[http://www.uniprot.org/uniprot/FLGA_SALTY FLGA_SALTY]] Involved in the assembly process of the P-ring formation. It may associate with FlgF on the rod constituting a structure essential for the P-ring assembly or may act as a modulator protein for the P-ring assembly. | [[http://www.uniprot.org/uniprot/FLGA_SALTY FLGA_SALTY]] Involved in the assembly process of the P-ring formation. It may associate with FlgF on the rod constituting a structure essential for the P-ring assembly or may act as a modulator protein for the P-ring assembly. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | A periplasmic flagellar chaperone protein, FlgA, is required for P-ring assembly in bacterial flagella of taxa such as Salmonella enterica or Escherichia coli. The mechanism of chaperone-mediated P-ring formation is poorly understood. Here we present the open and closed crystal structures of FlgA from Salmonella enterica serovar Typhimurium, grown under different crystallization conditions. An intramolecular disulfide cross-linked form of FlgA caused a dominant negative effect on motility of the wild-type strain. Pull-down experiments support a specific protein-protein interaction between FlgI, the P-ring component protein, and the C-terminal domain of FlgA. Surface plasmon resonance and limited-proteolysis indicate that flexibility of the domain is reduced in the covalently closed form. These results show that the structural flexibility of the C-terminal domain of FlgA, which is related to the structural difference between the two crystal forms, is intrinsically associated with its molecular chaperone function in P-ring assembly. | ||
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| + | Structural flexibility of the periplasmic protein, FlgA, regulates flagellar P-ring assembly in Salmonella enterica.,Matsunami H, Yoon YH, Meshcheryakov VA, Namba K, Samatey FA Sci Rep. 2016 Jun 7;6:27399. doi: 10.1038/srep27399. PMID:27273476<ref>PMID:27273476</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 3vjp" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Revision as of 17:29, 12 July 2016
Orthorhombic Crystal Structure of Salmonella FlgA in closed form
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