4ht9

From Proteopedia

(Difference between revisions)

Revision as of 12:06, 5 August 2016

Crystal structure of E coli Hfq bound to two RNAs

Structural highlights

4ht9 is a 5 chain structure with sequence from Ecobd. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	B21_04001, ECBD_3862, ECD_04039, hfq (ECOBD)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[C6ECV6_ECOBD] RNA chaperone that binds small regulatory RNA (sRNAs) and mRNAs to facilitate mRNA translational regulation in response to envelope stress, environmental stress and changes in metabolite concentrations. Also binds with high specificity to tRNAs (By similarity).[SAAS:SAAS005001_004_036087][HAMAP-Rule:MF_00436]

Publication Abstract from PubMed

The rpoS mRNA, which encodes the master regulator sigma(S) of general stress response, requires Hfq-facilitated base pairing with DsrA small RNA for efficient translation at low temperatures. It has recently been proposed that one mechanism underlying Hfq action is to bridge a transient ternary complex by simultaneously binding to rpoS and DsrA. However, no structural evidence of Hfq simultaneously bound to different RNAs has been reported. We detected simultaneous binding of Hfq to rpoS and DsrA fragments. Crystal structures of AU6A*Hfq*A7 and Hfq*A7 complexes were resolved using 1.8- and 1.9-A resolution, respectively. Ternary complex has been further verified in solution by NMR. In vivo, activation of rpoS translation requires intact Hfq, which is capable of bridging rpoS and DsrA simultaneously into ternary complex. This ternary complex possibly corresponds to a meta-stable transition state in Hfq-facilitated small RNA-mRNA annealing process.

Hfq-bridged ternary complex is important for translation activation of rpoS by DsrA.,Wang W, Wang L, Wu J, Gong Q, Shi Y Nucleic Acids Res. 2013 Jun 1;41(11):5938-48. doi: 10.1093/nar/gkt276. Epub 2013 , Apr 19. PMID:23605038^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Wang W, Wang L, Wu J, Gong Q, Shi Y. Hfq-bridged ternary complex is important for translation activation of rpoS by DsrA. Nucleic Acids Res. 2013 Jun 1;41(11):5938-48. doi: 10.1093/nar/gkt276. Epub 2013 , Apr 19. PMID:23605038 doi:10.1093/nar/gkt276

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4ht9"

@@ Line 1: / Line 1: @@
 ==Crystal structure of E coli Hfq bound to two RNAs==
 <StructureSection load='4ht9' size='340' side='right' caption='[[4ht9]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4ht9]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_bl21(de3) Escherichia coli bl21(de3)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HT9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HT9 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4ht9]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecobd Ecobd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HT9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HT9 FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">B21_04001, ECBD_3862, ECD_04039, hfq ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=469008 Escherichia coli BL21(DE3)])</td></tr>
+</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">B21_04001, ECBD_3862, ECD_04039, hfq ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=469008 ECOBD])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ht9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ht9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ht9 RCSB], [http://www.ebi.ac.uk/pdbsum/4ht9 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ht9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ht9 OCA], [http://pdbe.org/4ht9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ht9 RCSB], [http://www.ebi.ac.uk/pdbsum/4ht9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ht9 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 16: / Line 17: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4ht9" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>
 __TOC__
 </StructureSection>
+[[Category: Ecobd]]
 [[Category: Wang, L J]]
 [[Category: Wang, W W]]

4ht9

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Revision as of 12:06, 5 August 2016

Crystal structure of E coli Hfq bound to two RNAs

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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