4j6x

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(Difference between revisions)

Revision as of 12:45, 5 August 2016

Crystal structure of Hfq from Pseudomonas aeruginosa in complex with UTP

Structural highlights

4j6x is a 6 chain structure with sequence from Pseae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	1u1s, 1u1t, 3qui, 4j5y, 4j6w, 4j6y
Gene:	hfq, PA4944 (PSEAE)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[HFQ_PSEAE] RNA chaperone that binds small regulatory RNA (sRNAs) and mRNAs to facilitate mRNA translational regulation in response to envelope stress, environmental stress and changes in metabolite concentrations. Also binds with high specificity to tRNAs (By similarity).

Publication Abstract from PubMed

The Hfq protein forms a doughnut-shaped homohexamer that possesses RNA-binding activity. There are two distinct RNA-binding surfaces located on the proximal and the distal sides of the hexamer. The proximal side is involved in the binding of mRNA and small noncoding RNAs (sRNAs), while the distal side has an affinity for A-rich RNA sequences. In this work, the ability of various ribonucleotides to form complexes with Hfq from Pseudomonas aeruginosa has been tested using X-ray crystallography. ATP and ADPNP have been located in the distal R-site, which is a site for poly(A) RNA binding. UTP has been found in the so-called lateral RNA-binding site at the proximal surface. CTP has been found in both the distal R-site and the proximal U-binding site. GTP did not form a complex with Hfq under the conditions tested. The results have demonstrated the power of the crystallographic method for locating ribonucleotides and predicting single-stranded RNA-binding sites on the protein surface.

Hfq binds ribonucleotides in three different RNA-binding sites.,Murina V, Lekontseva N, Nikulin A Acta Crystallogr D Biol Crystallogr. 2013 Aug;69(Pt 8):1504-13. doi:, 10.1107/S090744491301010X. Epub 2013 Jul 18. PMID:23897473^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Murina V, Lekontseva N, Nikulin A. Hfq binds ribonucleotides in three different RNA-binding sites. Acta Crystallogr D Biol Crystallogr. 2013 Aug;69(Pt 8):1504-13. doi:, 10.1107/S090744491301010X. Epub 2013 Jul 18. PMID:23897473 doi:10.1107/S090744491301010X

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4j6x"

@@ Line 1: / Line 1: @@
 ==Crystal structure of Hfq from Pseudomonas aeruginosa in complex with UTP==
 <StructureSection load='4j6x' size='340' side='right' caption='[[4j6x]], [[Resolution|resolution]] 2.22&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4j6x]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_pao1 Pseudomonas aeruginosa pao1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J6X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4J6X FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4j6x]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J6X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4J6X FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=UTP:URIDINE+5-TRIPHOSPHATE'>UTP</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1u1s|1u1s]], [[1u1t|1u1t]], [[3qui|3qui]], [[4j5y|4j5y]], [[4j6w|4j6w]], [[4j6y|4j6y]]</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">hfq, PA4944 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 Pseudomonas aeruginosa PAO1])</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">hfq, PA4944 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4j6x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j6x OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4j6x RCSB], [http://www.ebi.ac.uk/pdbsum/4j6x PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4j6x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j6x OCA], [http://pdbe.org/4j6x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4j6x RCSB], [http://www.ebi.ac.uk/pdbsum/4j6x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4j6x ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 18: / Line 19: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4j6x" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Pseudomonas aeruginosa pao1]]
+[[Category: Pseae]]
 [[Category: Lekontseva, N]]
 [[Category: Murina, V]]

4j6x

From Proteopedia

Revision as of 12:45, 5 August 2016

Crystal structure of Hfq from Pseudomonas aeruginosa in complex with UTP

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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