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4h94

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Revision as of 12:55, 5 August 2016

Radiation damage in lysozyme - 0.56 MGy

Structural highlights

4h94 is a 1 chain structure with sequence from Chick. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Related:	4h8x, 4h8y, 4h8z, 4h90, 4h91, 4h92, 4h93, 4h9a, 4h9b, 4h9c, 4h9e, 4h9f, 4h9h, 4h9i
Activity:	Lysozyme, with EC number 3.2.1.17
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[LYSC_CHICK] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.^[1]

Publication Abstract from PubMed

Electron paramagnetic resonance (EPR) and online UV-visible absorption microspectrophotometry with X-ray crystallography have been used in a complementary manner to follow X-ray-induced disulfide-bond cleavage. Online UV-visible spectroscopy showed that upon X-irradiation, disulfide radicalization appeared to saturate at an absorbed dose of approximately 0.5-0.8 MGy, in contrast to the saturating dose of approximately 0.2 MGy observed using EPR at much lower dose rates. The observations suggest that a multi-track model involving product formation owing to the interaction of two separate tracks is a valid model for radiation damage in protein crystals. The saturation levels are remarkably consistent given the widely different experimental parameters and the range of total absorbed doses studied. The results indicate that even at the lowest doses used for structural investigations disulfide bonds are already radicalized. Multi-track considerations offer the first step in a comprehensive model of radiation damage that could potentially lead to a combined computational and experimental approach to identifying when damage is likely to be present, to quantitate it and to provide the ability to recover the native unperturbed structure.

Insights into the mechanism of X-ray-induced disulfide-bond cleavage in lysozyme crystals based on EPR, optical absorption and X-ray diffraction studies.,Sutton KA, Black PJ, Mercer KR, Garman EF, Owen RL, Snell EH, Bernhard WA Acta Crystallogr D Biol Crystallogr. 2013 Dec;69(Pt 12):2381-94. doi:, 10.1107/S0907444913022117. Epub 2013 Nov 19. PMID:24311579^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Maehashi K, Matano M, Irisawa T, Uchino M, Kashiwagi Y, Watanabe T. Molecular characterization of goose- and chicken-type lysozymes in emu (Dromaius novaehollandiae): evidence for extremely low lysozyme levels in emu egg white. Gene. 2012 Jan 15;492(1):244-9. doi: 10.1016/j.gene.2011.10.021. Epub 2011 Oct, 25. PMID:22044478 doi:10.1016/j.gene.2011.10.021
↑ Sutton KA, Black PJ, Mercer KR, Garman EF, Owen RL, Snell EH, Bernhard WA. Insights into the mechanism of X-ray-induced disulfide-bond cleavage in lysozyme crystals based on EPR, optical absorption and X-ray diffraction studies. Acta Crystallogr D Biol Crystallogr. 2013 Dec;69(Pt 12):2381-94. doi:, 10.1107/S0907444913022117. Epub 2013 Nov 19. PMID:24311579 doi:http://dx.doi.org/10.1107/S0907444913022117

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4h94"

Categories: Chick | Lysozyme | Snell, E H | Sutton, K A | Hydrolase

@@ Line 1: / Line 1: @@
 ==Radiation damage in lysozyme - 0.56 MGy==
 <StructureSection load='4h94' size='340' side='right' caption='[[4h94]], [[Resolution|resolution]] 1.20&Aring;' scene=''>
@@ Line 6: / Line 7: @@
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4h8x|4h8x]], [[4h8y|4h8y]], [[4h8z|4h8z]], [[4h90|4h90]], [[4h91|4h91]], [[4h92|4h92]], [[4h93|4h93]], [[4h9a|4h9a]], [[4h9b|4h9b]], [[4h9c|4h9c]], [[4h9e|4h9e]], [[4h9f|4h9f]], [[4h9h|4h9h]], [[4h9i|4h9i]]</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4h94 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h94 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4h94 RCSB], [http://www.ebi.ac.uk/pdbsum/4h94 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4h94 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h94 OCA], [http://pdbe.org/4h94 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4h94 RCSB], [http://www.ebi.ac.uk/pdbsum/4h94 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4h94 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 18: / Line 19: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4h94" style="background-color:#fffaf0;"></div>
 ==See Also==

4h94

From Proteopedia

Revision as of 12:55, 5 August 2016

Radiation damage in lysozyme - 0.56 MGy

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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