2exa
From Proteopedia
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|PDB= 2exa |SIZE=350|CAPTION= <scene name='initialview01'>2exa</scene>, resolution 1.70Å | |PDB= 2exa |SIZE=350|CAPTION= <scene name='initialview01'>2exa</scene>, resolution 1.70Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=FRO:(5R,6S)-6-[(1R)-1-HYDROXYETHYL]-7-OXO-3-[(2R)-TETRAHYDROFURAN-2-YL]-4-THIA-1-AZABICYCLO[3.2.0]HEPT-2-ENE-2-CARBOXYLATE'>FRO</scene> | + | |LIGAND= <scene name='pdbligand=FRO:(5R,6S)-6-[(1R)-1-HYDROXYETHYL]-7-OXO-3-[(2R)-TETRAHYDROFURAN-2-YL]-4-THIA-1-AZABICYCLO[3.2.0]HEPT-2-ENE-2-CARBOXYLATE'>FRO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= dacb ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | |GENE= dacb ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[2ex2|2EX2]], [[2ex6|2EX6]], [[2ex8|2EX8]], [[2ex9|2EX9]], [[2exb|2EXB]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2exa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2exa OCA], [http://www.ebi.ac.uk/pdbsum/2exa PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2exa RCSB]</span> | ||
}} | }} | ||
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[[Category: Tame, J R.H.]] | [[Category: Tame, J R.H.]] | ||
[[Category: Unzai, S.]] | [[Category: Unzai, S.]] | ||
| - | [[Category: FRO]] | ||
| - | [[Category: GOL]] | ||
| - | [[Category: cephem]] | ||
[[Category: d-alanyl-d-alanine-carboxypeptidase]] | [[Category: d-alanyl-d-alanine-carboxypeptidase]] | ||
[[Category: d-alanyl-d-alanine-endopeptidase]] | [[Category: d-alanyl-d-alanine-endopeptidase]] | ||
[[Category: farom]] | [[Category: farom]] | ||
[[Category: penem]] | [[Category: penem]] | ||
| - | [[Category: penicillin]] | + | [[Category: penicillin,cephem]] |
[[Category: penicillin-binding protein]] | [[Category: penicillin-binding protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:55:00 2008'' |
Revision as of 23:55, 30 March 2008
| |||||||
| , resolution 1.70Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Gene: | dacb (Escherichia coli) | ||||||
| Related: | 2EX2, 2EX6, 2EX8, 2EX9, 2EXB
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of penicillin binding protein 4 (dacB) from Escherichia coli, complexed with FAROM
Overview
The crystal structure of penicillin binding protein 4 (PBP4) from Escherichia coli, which has both DD-endopeptidase and DD-carboxypeptidase activity, is presented. PBP4 is one of 12 penicillin binding proteins in E. coli involved in the synthesis and maintenance of the cell wall. The model contains a penicillin binding domain similar to known structures, but includes a large insertion which folds into domains with unique folds. The structures of the protein covalently attached to five different antibiotics presented here show the active site residues are unmoved compared to the apoprotein, but nearby surface loops and helices are displaced in some cases. The altered geometry of conserved active site residues compared with those of other PBPs suggests a possible cause for the slow deacylation rate of PBP4.
About this Structure
2EXA is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Crystal structure of penicillin binding protein 4 (dacB) from Escherichia coli, both in the native form and covalently linked to various antibiotics., Kishida H, Unzai S, Roper DI, Lloyd A, Park SY, Tame JR, Biochemistry. 2006 Jan 24;45(3):783-92. PMID:16411754
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