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4a7e

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Revision as of 14:50, 5 August 2016

X-ray crystal structure of porcine insulin flash-cooled at high pressure

Structural highlights

4a7e is a 2 chain structure with sequence from Sus scrofa. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	1zei, 1b2g, 3ins, 1wav, 1b2b, 1b2f, 1m5a, 9ins, 1b2d, 1dei, 1b17, 1sdb, 1b2a, 1izb, 2tci, 1b2c, 4ins, 7ins, 1iza, 3mth, 1b19, 1mpj, 1zni, 1b2e, 1b18, 4a7d
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[INS_PIG] Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

Publication Abstract from PubMed

High-pressure freezing (HPF) is a method which allows sample vitrification without cryoprotectants. In the present work, protein crystals were cooled to cryogenic temperatures at a pressure of 210 MPa. In contrast to other HPF methods published to date in the field of cryocrystallography, this protocol involves rapid sample cooling using a standard HPF device. The fast cooling rates allow HPF of protein crystals directly in their mother liquor without the need for cryoprotectants or external reagents. HPF was first attempted with hen egg-white lysozyme and cubic insulin crystals, yielding good to excellent diffraction quality. Non-cryoprotected crystals of the membrane protein photosystem II have been successfully cryocooled for the first time. This indicates that the presented HPF method is well suited to the vitrification of challenging systems with large unit cells and weak crystal contacts.

Fast high-pressure freezing of protein crystals in their mother liquor.,Burkhardt A, Warmer M, Panneerselvam S, Wagner A, Zouni A, Glockner C, Reimer R, Hohenberg H, Meents A Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Apr 1;68(Pt 4):495-500., Epub 2012 Mar 31. PMID:22505429^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Burkhardt A, Warmer M, Panneerselvam S, Wagner A, Zouni A, Glockner C, Reimer R, Hohenberg H, Meents A. Fast high-pressure freezing of protein crystals in their mother liquor. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Apr 1;68(Pt 4):495-500., Epub 2012 Mar 31. PMID:22505429 doi:10.1107/S1744309112009670

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4a7e"

@@ Line 1: / Line 1: @@
 ==X-ray crystal structure of porcine insulin flash-cooled at high pressure==
 <StructureSection load='4a7e' size='340' side='right' caption='[[4a7e]], [[Resolution|resolution]] 1.86&Aring;' scene=''>
@@ Line 4: / Line 5: @@
 <table><tr><td colspan='2'>[[4a7e]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A7E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4A7E FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1zei|1zei]], [[1b2g|1b2g]], [[3ins|3ins]], [[1wav|1wav]], [[1b2b|1b2b]], [[1b2f|1b2f]], [[1m5a|1m5a]], [[9ins|9ins]], [[1b2d|1b2d]], [[1dei|1dei]], [[1b17|1b17]], [[1sdb|1sdb]], [[1b2a|1b2a]], [[1izb|1izb]], [[2tci|2tci]], [[1b2c|1b2c]], [[4ins|4ins]], [[7ins|7ins]], [[1iza|1iza]], [[3mth|3mth]], [[1b19|1b19]], [[1mpj|1mpj]], [[1zni|1zni]], [[1b2e|1b2e]], [[1b18|1b18]], [[4a7d|4a7d]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4a7e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a7e OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4a7e RCSB], [http://www.ebi.ac.uk/pdbsum/4a7e PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4a7e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a7e OCA], [http://pdbe.org/4a7e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4a7e RCSB], [http://www.ebi.ac.uk/pdbsum/4a7e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4a7e ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 16: / Line 17: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4a7e" style="background-color:#fffaf0;"></div>
 ==See Also==

4a7e

From Proteopedia

Revision as of 14:50, 5 August 2016

X-ray crystal structure of porcine insulin flash-cooled at high pressure

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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