3vzg

From Proteopedia

(Difference between revisions)

Revision as of 15:38, 5 August 2016

Crystal structure of human pancreatic secretory protein ZG16p with O-(alpha-D-mannosyl)-L-threonine

Structural highlights

3vzg is a 1 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, ,
Related:	3vzf, 3vze
Gene:	ZG16 (HUMAN)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[ZG16_HUMAN] May play a role in protein trafficking. May act as a linker molecule between the submembranous matrix on the luminal side of zymogen granule membrane (ZGM) and aggregated secretory proteins during granule formation in the TGN.^[1]

Publication Abstract from PubMed

ZG16p is a soluble mammalian lectin, the first to be described with a Jacalin-related beta-prism-fold. ZG16p has been reported to bind both to glycosaminoglycans and mannose. To determine the structural basis of the multiple sugar-binding properties, we conducted glycan microarray analyses of human ZG16p. We observed that ZG16p preferentially binds to alpha-mannose-terminating short glycans such as Ser/Thr-linked O-mannose, but not to high mannose-type N-glycans. Among sulfated glycosaminoglycan oligomers examined, chondroitin sulfate B and heparin oligosaccharides showed significant binding. Crystallographic studies of human ZG16p lectin in the presence of selected ligands revealed the mechanism of multiple sugar recognition. Manalpha1-3Man and Glcbeta1-3Glc bound in different orientations: the nonreducing end of the former and the reducing end of the latter fitted in the canonical shallow mannose binding pocket. Solution NMR analysis using (15)N-labeled ZG16p defined the heparin-binding region, which is on an adjacent flat surface of the protein. On-array competitive binding assays suggest that it is possible for ZG16p to bind simultaneously to both types of ligands. Recognition of a broad spectrum of ligands by ZG16p may account for the multiple functions of this lectin in the formation of zymogen granules via glycosaminoglycan binding, and in the recognition of pathogens in the digestive system through alpha-mannose-related recognition.

Structural Basis for Multiple Sugar Recognition of Jacalin-related Human ZG16p Lectin.,Kanagawa M, Liu Y, Hanashima S, Ikeda A, Chai W, Nakano Y, Kojima-Aikawa K, Feizi T, Yamaguchi Y J Biol Chem. 2014 Jun 13;289(24):16954-65. doi: 10.1074/jbc.M113.539114. Epub, 2014 Apr 30. PMID:24790092^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Zhou YB, Cao JB, Yang HM, Zhu H, Xu ZG, Wang KS, Zhang X, Wang ZQ, Han ZG. hZG16, a novel human secreted protein expressed in liver, was down-regulated in hepatocellular carcinoma. Biochem Biophys Res Commun. 2007 Apr 13;355(3):679-86. Epub 2007 Feb 12. PMID:17307141 doi:10.1016/j.bbrc.2007.02.020
↑ Kanagawa M, Liu Y, Hanashima S, Ikeda A, Chai W, Nakano Y, Kojima-Aikawa K, Feizi T, Yamaguchi Y. Structural Basis for Multiple Sugar Recognition of Jacalin-related Human ZG16p Lectin. J Biol Chem. 2014 Jun 13;289(24):16954-65. doi: 10.1074/jbc.M113.539114. Epub, 2014 Apr 30. PMID:24790092 doi:http://dx.doi.org/10.1074/jbc.M113.539114

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3vzg"

Categories: Human | Kanagawa, M | Yamaguchi, Y | Beta-prism fold | Sugar binding protein

@@ Line 1: / Line 1: @@
 ==Crystal structure of human pancreatic secretory protein ZG16p with O-(alpha-D-mannosyl)-L-threonine==
 <StructureSection load='3vzg' size='340' side='right' caption='[[3vzg]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
@@ Line 6: / Line 7: @@
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3vzf|3vzf]], [[3vze|3vze]]</td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ZG16 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vzg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vzg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vzg RCSB], [http://www.ebi.ac.uk/pdbsum/3vzg PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vzg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vzg OCA], [http://pdbe.org/3vzg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3vzg RCSB], [http://www.ebi.ac.uk/pdbsum/3vzg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3vzg ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 18: / Line 19: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 3vzg" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>

3vzg

From Proteopedia

Revision as of 15:38, 5 August 2016

Crystal structure of human pancreatic secretory protein ZG16p with O-(alpha-D-mannosyl)-L-threonine

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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