2fco
From Proteopedia
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|PDB= 2fco |SIZE=350|CAPTION= <scene name='initialview01'>2fco</scene>, resolution 1.40Å | |PDB= 2fco |SIZE=350|CAPTION= <scene name='initialview01'>2fco</scene>, resolution 1.40Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Crossover_junction_endodeoxyribonuclease Crossover junction endodeoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.22.4 3.1.22.4] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Crossover_junction_endodeoxyribonuclease Crossover junction endodeoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.22.4 3.1.22.4] </span> |
|GENE= RecU/PrfA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1462 Geobacillus kaustophilus]) | |GENE= RecU/PrfA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1462 Geobacillus kaustophilus]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fco FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fco OCA], [http://www.ebi.ac.uk/pdbsum/2fco PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2fco RCSB]</span> | ||
}} | }} | ||
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[[Category: Jedrzejas, M J.]] | [[Category: Jedrzejas, M J.]] | ||
[[Category: Li, J.]] | [[Category: Li, J.]] | ||
| - | [[Category: EDO]] | ||
| - | [[Category: MG]] | ||
[[Category: crystal structure]] | [[Category: crystal structure]] | ||
[[Category: flexibility]] | [[Category: flexibility]] | ||
[[Category: hydrolase]] | [[Category: hydrolase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:00:58 2008'' |
Revision as of 00:00, 31 March 2008
| |||||||
| , resolution 1.40Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Gene: | RecU/PrfA (Geobacillus kaustophilus) | ||||||
| Activity: | Crossover junction endodeoxyribonuclease, with EC number 3.1.22.4 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of Bacillus stearothermophilus PrfA-Holliday Junction Resolvase
Overview
Here we report a high resolution structure of RecU-Holliday junction resolvase from Bacillus stearothermophilus. The functional unit of RecU is a homodimer that contains a "mushroom" like structure with a rigid cap and two highly flexible loops extending outwards. These loops appear to be highly flexible/dynamic, and presumably are directly involved in DNA binding and holding it for catalysis. Structural modifications of both the protein and DNA upon their interaction are essential for catalysis. An Mg2+ ion is present in each of the two active sites in this homodimeric enzyme, and two water molecules are coordinated with each Mg2+ ion. Our data are consistent with one of these water molecules acting as a nucleophile and the other as a general acid. The identities of the general base and general acid involved in catalysis and the Lewis acid that stabilizes the pentacovalent transition state phosphate ion are proposed. A model for the RecU-Holliday junction DNA complex is also proposed and discussed in the context of DNA binding and cleavage.
About this Structure
2FCO is a Single protein structure of sequence from Geobacillus kaustophilus. Full crystallographic information is available from OCA.
Reference
Structure, flexibility, and mechanism of the Bacillus stearothermophilus RecU Holliday junction resolvase., Kelly SJ, Li J, Setlow P, Jedrzejas MJ, Proteins. 2007 Sep 1;68(4):961-71. PMID:17557334
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