4a5v

From Proteopedia

(Difference between revisions)

Revision as of 19:46, 5 August 2016

Solution structure ensemble of the two N-terminal apple domains (residues 58-231) of Toxoplasma gondii microneme protein 4

Structural highlights

4a5v is a 1 chain structure with sequence from Toxoplasma gondii rh. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[MIC4_TOXGO] Adhesin. Required for attachment of the parasite to the host cell prior to invasion.^[1] ^[2]

Publication Abstract from PubMed

Toxosplasma gondii is the model parasite of the phylum Apicomplexa, which contains numerous obligate intracellular parasites of medical and veterinary importance, including Eimeria, Sarcocystis, Cryptosporidium, Cyclospora and Plasmodium species. Members of this phylum actively enter host cells by a multi-step process with the help of microneme protein (MIC) complexes that play important roles in motility, host cell attachment, moving junction formation and invasion. Toxoplasma gondii (Tg)MIC1-4-6 complex is the most extensively investigated microneme complex which contributes to host cell recognition and attachment via the action of TgMIC1, a sialic acid-binding adhesin. Here, we report the structure of TgMIC4 and reveal its carbohydrate-binding specificity to a variety of galactose-containing carbohydrate ligands. The lectin is composed of six apple domains in which the fifth domain displays a potent galactose-binding activity, and which is cleaved from the complex during parasite invasion. We propose that galactose recognition by TgMIC4 masks the recognition of the parasite thereby providing protection from galectin-mediated activation of the host immune system.

Galactose recognition by the apicomplexan parasite Toxoplasma gondii.,Marchant J, Cowper B, Liu Y, Lai L, Pinzan C, Marq JB, Friedrich N, Sawmynaden K, Liew L, Chai W, Childs RA, Saouros S, Simpson P, Roque Barreira MC, Feizi T, Soldati-Favre D, Matthews S J Biol Chem. 2012 Mar 7. PMID:22399295^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Brecht S, Carruthers VB, Ferguson DJ, Giddings OK, Wang G, Jakle U, Harper JM, Sibley LD, Soldati D. The toxoplasma micronemal protein MIC4 is an adhesin composed of six conserved apple domains. J Biol Chem. 2001 Feb 9;276(6):4119-27. Epub 2000 Oct 26. PMID:11053441 doi:http://dx.doi.org/10.1074/jbc.M008294200
↑ Reiss M, Viebig N, Brecht S, Fourmaux MN, Soete M, Di Cristina M, Dubremetz JF, Soldati D. Identification and characterization of an escorter for two secretory adhesins in Toxoplasma gondii. J Cell Biol. 2001 Feb 5;152(3):563-78. PMID:11157983
↑ Marchant J, Cowper B, Liu Y, Lai L, Pinzan C, Marq JB, Friedrich N, Sawmynaden K, Liew L, Chai W, Childs RA, Saouros S, Simpson P, Roque Barreira MC, Feizi T, Soldati-Favre D, Matthews S. Galactose recognition by the apicomplexan parasite Toxoplasma gondii. J Biol Chem. 2012 Mar 7. PMID:22399295 doi:10.1074/jbc.M111.325928

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4a5v"

@@ Line 1: / Line 1: @@
 ==Solution structure ensemble of the two N-terminal apple domains (residues 58-231) of Toxoplasma gondii microneme protein 4==
 <StructureSection load='4a5v' size='340' side='right' caption='[[4a5v]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4a5v]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Toxoplasma_gondii Toxoplasma gondii]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A5V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4A5V FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4a5v]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Toxoplasma_gondii_rh Toxoplasma gondii rh]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A5V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4A5V FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4a5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a5v OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4a5v RCSB], [http://www.ebi.ac.uk/pdbsum/4a5v PDBsum]</span></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4a5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a5v OCA], [http://pdbe.org/4a5v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4a5v RCSB], [http://www.ebi.ac.uk/pdbsum/4a5v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4a5v ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 15: / Line 16: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4a5v" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Toxoplasma gondii]]
+[[Category: Toxoplasma gondii rh]]
 [[Category: Barreira, M C.R]]
 [[Category: Chai, W]]

4a5v

From Proteopedia

Revision as of 19:46, 5 August 2016

Solution structure ensemble of the two N-terminal apple domains (residues 58-231) of Toxoplasma gondii microneme protein 4

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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