2fio
From Proteopedia
| Line 4: | Line 4: | ||
|PDB= 2fio |SIZE=350|CAPTION= <scene name='initialview01'>2fio</scene>, resolution 2.700Å | |PDB= 2fio |SIZE=350|CAPTION= <scene name='initialview01'>2fio</scene>, resolution 2.700Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= | + | |LIGAND= <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene> |
|ACTIVITY= | |ACTIVITY= | ||
| - | |GENE= 4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id= | + | |GENE= 4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10756 Bacillus phage phi29]) |
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fio FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fio OCA], [http://www.ebi.ac.uk/pdbsum/2fio PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2fio RCSB]</span> | ||
}} | }} | ||
| Line 16: | Line 19: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2FIO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ | + | 2FIO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_phage_phi29 Bacillus phage phi29]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FIO OCA]. |
==Reference== | ==Reference== | ||
The structure of phage phi29 transcription regulator p4-DNA complex reveals an N-hook motif for DNA., Badia D, Camacho A, Perez-Lago L, Escandon C, Salas M, Coll M, Mol Cell. 2006 Apr 7;22(1):73-81. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16600871 16600871] | The structure of phage phi29 transcription regulator p4-DNA complex reveals an N-hook motif for DNA., Badia D, Camacho A, Perez-Lago L, Escandon C, Salas M, Coll M, Mol Cell. 2006 Apr 7;22(1):73-81. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16600871 16600871] | ||
| + | [[Category: Bacillus phage phi29]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Vibrio phage f237]] | ||
[[Category: Badia, D.]] | [[Category: Badia, D.]] | ||
[[Category: Camacho, A.]] | [[Category: Camacho, A.]] | ||
| Line 28: | Line 31: | ||
[[Category: Perez-Lago, L.]] | [[Category: Perez-Lago, L.]] | ||
[[Category: Salas, M.]] | [[Category: Salas, M.]] | ||
| - | [[Category: | + | [[Category: n-hook dna-binding motif]] |
| + | [[Category: protein-dna complex]] | ||
| + | [[Category: transcription regulation]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:03:18 2008'' |
Revision as of 00:03, 31 March 2008
| |||||||
| , resolution 2.700Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , | ||||||
| Gene: | 4 (Bacillus phage phi29) | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Phage phi29 transcription regulator p4-DNA complex
Overview
Protein p4 affects the transcriptional switch that divides bacteriophage phi29 infection in early and late phases. The synthesis of DNA replication proteins and p4 takes place in the early phase, while structural, morphogenesis, and lysis proteins are synthesized in the late phase. Transcriptional switch by p4 is achieved by activating the late promoter A3 and repressing the early promoters A2b and A2c. The crystal structure of p4 alone and in complex with a 41 bp DNA, including the A3 promoter binding site, helps us to understand how the phage cycle is controlled. Protein p4 has a unique alpha/beta fold that includes a DNA recognition motif consisting of two N-terminal beta turn substructures, or N-hooks, located at the tips of an elongated protein homodimer. The two N-hooks enter the major groove of the double helix, establishing base-specific contacts. A high DNA curvature allows p4 N-hooks to reach two major groove areas three helical turns apart, like a bow and its string.
About this Structure
2FIO is a Single protein structure of sequence from Bacillus phage phi29. Full crystallographic information is available from OCA.
Reference
The structure of phage phi29 transcription regulator p4-DNA complex reveals an N-hook motif for DNA., Badia D, Camacho A, Perez-Lago L, Escandon C, Salas M, Coll M, Mol Cell. 2006 Apr 7;22(1):73-81. PMID:16600871
Page seeded by OCA on Mon Mar 31 03:03:18 2008
