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2fl1

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|PDB= 2fl1 |SIZE=350|CAPTION= <scene name='initialview01'>2fl1</scene>, resolution 2.4&Aring;
|PDB= 2fl1 |SIZE=350|CAPTION= <scene name='initialview01'>2fl1</scene>, resolution 2.4&Aring;
|SITE=
|SITE=
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|LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene>
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|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=XYG:[(4Z)-2-[(1Z)-ETHANIMIDOYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>XYG</scene>
|ACTIVITY=
|ACTIVITY=
|GENE=
|GENE=
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|DOMAIN=
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|RELATEDENTRY=[[1xa9|1XA9]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fl1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fl1 OCA], [http://www.ebi.ac.uk/pdbsum/2fl1 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2fl1 RCSB]</span>
}}
}}
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[[Category: Popov, B.]]
[[Category: Popov, B.]]
[[Category: Tikhonova, T.]]
[[Category: Tikhonova, T.]]
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[[Category: SO4]]
 
[[Category: beta barrel]]
[[Category: beta barrel]]
[[Category: beta-can fold]]
[[Category: beta-can fold]]
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[[Category: zrfp574]]
[[Category: zrfp574]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:53:39 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:04:12 2008''

Revision as of 00:04, 31 March 2008

Image:2fl1.jpg


PDB ID 2fl1

Drag the structure with the mouse to rotate
, resolution 2.4Å
Ligands: ,
Related: 1XA9


Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



Crystal structure of red fluorescent protein from Zoanthus, zRFP574, at 2.4A resolution


Overview

The three-dimensional structure of the red fluorescent protein (RFP) zRFP574 from the button polyp Zoanthus sp. (two dimers per asymmetric unit, 231 x 4 amino acids) has been determined at 2.4 A resolution in space group C222(1). The crystal structure, refined to a crystallographic R factor of 0.203 (R(free) = 0.249), adopts the beta-barrel fold composed of 11 strands similar to that of the yellow fluorescent protein zYFP538. The zRFP574 chromophore, originating from the protein sequence Asp66-Tyr67-Gly68, has a two-ring structure typical of GFP-like proteins. The bond geometry of residue 66 shows the strong tendency of the corresponding C(alpha) atom to sp(2) hybridization as a consequence of N-acylimine bond formation. The zRFP574 chromophore contains the 65-66 cis-peptide bond characteristic of red fluorescent proteins. The chromophore phenolic ring adopts a cis conformation coplanar with the imidazolinone ring. The crystallographic study has revealed an unexpected chemical feature of the internal chromophore. A decarboxylated side chain of the chromophore-forming residue Asp66 has been observed in the structure. This additional post-translational modification is likely to play a key role in the bathochromic shift of the zRFP574 spectrum.

About this Structure

2FL1 is a Single protein structure of sequence from Zoanthus sp.. Full crystallographic information is available from OCA.

Reference

Structure of a red fluorescent protein from Zoanthus, zRFP574, reveals a novel chromophore., Pletneva N, Pletnev S, Tikhonova T, Popov V, Martynov V, Pletnev V, Acta Crystallogr D Biol Crystallogr. 2006 May;62(Pt 5):527-32. Epub 2006, Apr 19. PMID:16627946

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