4kop

From Proteopedia

(Difference between revisions)

Revision as of 22:43, 5 August 2016

Crystal Structure of WHY2 from Arabidopsis thaliana

Structural highlights

4kop is a 4 chain structure with sequence from Arath. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	4koo, 4koq
Gene:	At1g71260, F3I17.9, WHY2 (ARATH)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[WHY2_ARATH] Single-stranded DNA-binding protein that associates with mitochondrial DNA and may play a role in the regulation of the gene expression machinery. Seems also to be required to prevent break-induced DNA rearrangements in the mitochondrial genome. Can bind to melt double-stranded DNA in vivo.^[1] ^[2] ^[3]

Publication Abstract from PubMed

DNA double-strand breaks are highly detrimental genomic lesions that routinely arise in genomes. To protect the integrity of their genetic information, all organisms have evolved specialized DNA-repair mechanisms. Whirly proteins modulate DNA repair in plant chloroplasts and mitochondria by binding single-stranded DNA in a non-sequence-specific manner. Although most of the results showing the involvement of the Whirly proteins in DNA repair have been obtained in Arabidopsis thaliana, only the crystal structures of the potato Whirly proteins WHY1 and WHY2 have been reported to date. The present report of the crystal structures of the three Whirly proteins from A. thaliana (WHY1, WHY2 and WHY3) reveals that these structurally similar proteins assemble into tetramers. Furthermore, structural alignment with a potato WHY2-DNA complex reveals that the residues in these proteins are properly oriented to bind single-stranded DNA in a non-sequence-specific manner.

A family portrait: structural comparison of the Whirly proteins from Arabidopsis thaliana and Solanum tuberosum.,Cappadocia L, Parent JS, Sygusch J, Brisson N Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Nov;69(Pt 11):1207-11., doi: 10.1107/S1744309113028698. Epub 2013 Oct 26. PMID:24192350^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Marechal A, Parent JS, Sabar M, Veronneau-Lafortune F, Abou-Rached C, Brisson N. Overexpression of mtDNA-associated AtWhy2 compromises mitochondrial function. BMC Plant Biol. 2008 Apr 18;8:42. doi: 10.1186/1471-2229-8-42. PMID:18423020 doi:http://dx.doi.org/10.1186/1471-2229-8-42
↑ Cappadocia L, Marechal A, Parent JS, Lepage E, Sygusch J, Brisson N. Crystal structures of DNA-Whirly complexes and their role in Arabidopsis organelle genome repair. Plant Cell. 2010 Jun;22(6):1849-67. Epub 2010 Jun 15. PMID:20551348 doi:10.1105/tpc.109.071399
↑ Janicka S, Kuhn K, Le Ret M, Bonnard G, Imbault P, Augustyniak H, Gualberto JM. A RAD52-like single-stranded DNA binding protein affects mitochondrial DNA repair by recombination. Plant J. 2012 Nov;72(3):423-35. doi: 10.1111/j.1365-313X.2012.05097.x. Epub 2012 , Aug 30. PMID:22762281 doi:http://dx.doi.org/10.1111/j.1365-313X.2012.05097.x
↑ Cappadocia L, Parent JS, Sygusch J, Brisson N. A family portrait: structural comparison of the Whirly proteins from Arabidopsis thaliana and Solanum tuberosum. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Nov;69(Pt 11):1207-11., doi: 10.1107/S1744309113028698. Epub 2013 Oct 26. PMID:24192350 doi:http://dx.doi.org/10.1107/S1744309113028698

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4kop"

@@ Line 1: / Line 1: @@
 ==Crystal Structure of WHY2 from Arabidopsis thaliana==
 <StructureSection load='4kop' size='340' side='right' caption='[[4kop]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
@@ Line 6: / Line 7: @@
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4koo|4koo]], [[4koq|4koq]]</td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">At1g71260, F3I17.9, WHY2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kop FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kop OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4kop RCSB], [http://www.ebi.ac.uk/pdbsum/4kop PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kop FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kop OCA], [http://pdbe.org/4kop PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4kop RCSB], [http://www.ebi.ac.uk/pdbsum/4kop PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4kop ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 18: / Line 19: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4kop" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>

4kop

From Proteopedia

Revision as of 22:43, 5 August 2016

Crystal Structure of WHY2 from Arabidopsis thaliana

Structural highlights

Function

Publication Abstract from PubMed

References

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Proteopedia Page Contributors and Editors (what is this?)

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