2ft0
From Proteopedia
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|PDB= 2ft0 |SIZE=350|CAPTION= <scene name='initialview01'>2ft0</scene>, resolution 1.66Å | |PDB= 2ft0 |SIZE=350|CAPTION= <scene name='initialview01'>2ft0</scene>, resolution 1.66Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=ACO:ACETYL COENZYME *A'>ACO</scene> | + | |LIGAND= <scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= wecd ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | |GENE= wecd ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[2fs5|2FS5]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ft0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ft0 OCA], [http://www.ebi.ac.uk/pdbsum/2ft0 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ft0 RCSB]</span> | ||
}} | }} | ||
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[[Category: Rangarajan, E.]] | [[Category: Rangarajan, E.]] | ||
[[Category: Sulea, T.]] | [[Category: Sulea, T.]] | ||
| - | [[Category: ACO]] | ||
[[Category: bsgi]] | [[Category: bsgi]] | ||
[[Category: gnat fold acetyltransferase]] | [[Category: gnat fold acetyltransferase]] | ||
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[[Category: structural genomic]] | [[Category: structural genomic]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:07:19 2008'' |
Revision as of 00:07, 31 March 2008
| |||||||
| , resolution 1.66Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | wecd (Escherichia coli) | ||||||
| Related: | 2FS5
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of TDP-fucosamine acetyltransferase (WecD)- complex with acetyl-CoA
Overview
Enterobacterial common antigen (ECA) is a polysaccharide found on the outer membrane of virtually all gram-negative enteric bacteria and consists of three sugars, N-acetyl-d-glucosamine, N-acetyl-d-mannosaminuronic acid, and 4-acetamido-4,6-dideoxy-d-galactose, organized into trisaccharide repeating units having the sequence -->3)-alpha-d-Fuc4NAc-(1-->4)-beta-d-ManNAcA-(1-->4)-alpha-d-GlcNAc-(1-->. While the precise function of ECA is unknown, it has been linked to the resistance of Shiga-toxin-producing Escherichia coli (STEC) O157:H7 to organic acids and the resistance of Salmonella enterica to bile salts. The final step in the synthesis of 4-acetamido-4,6-dideoxy-d-galactose, the acetyl-coenzyme A (CoA)-dependent acetylation of the 4-amino group, is carried out by TDP-fucosamine acetyltransferase (WecD). We have determined the crystal structure of WecD in apo form at a 1.95-Angstrom resolution and bound to acetyl-CoA at a 1.66-Angstrom resolution. WecD is a dimeric enzyme, with each monomer adopting the GNAT N-acetyltransferase fold, common to a number of enzymes involved in acetylation of histones, aminoglycoside antibiotics, serotonin, and sugars. The crystal structure of WecD, however, represents the first structure of a GNAT family member that acts on nucleotide sugars. Based on this cocrystal structure, we have used flexible docking to generate a WecD-bound model of the acetyl-CoA-TDP-fucosamine tetrahedral intermediate, representing the structure during acetyl transfer. Our structural data show that WecD does not possess a residue that directly functions as a catalytic base, although Tyr208 is well positioned to function as a general acid by protonating the thiolate anion of coenzyme A.
About this Structure
2FT0 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Crystal structure of TDP-fucosamine acetyltransferase (WecD) from Escherichia coli, an enzyme required for enterobacterial common antigen synthesis., Hung MN, Rangarajan E, Munger C, Nadeau G, Sulea T, Matte A, J Bacteriol. 2006 Aug;188(15):5606-17. PMID:16855251
Page seeded by OCA on Mon Mar 31 03:07:19 2008
Categories: Escherichia coli | Single protein | BSGI, Montreal-Kingston Bacterial Structural Genomics Initiative. | Hung, M N. | Matte, A. | Munger, C. | Nadeau, G. | Rangarajan, E. | Sulea, T. | Bsgi | Gnat fold acetyltransferase | Montreal-kingston bacterial structural genomics initiative | Structural genomic
