4ofj
From Proteopedia
(Difference between revisions)
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| - | ''' | + | ==Crystal structure of NikA from Staphylococcus aureus in complex with Ni(L-His)2== |
| + | <StructureSection load='4ofj' size='340' side='right' caption='[[4ofj]], [[Resolution|resolution]] 1.70Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4ofj]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OFJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OFJ FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HIS:HISTIDINE'>HIS</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ofj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ofj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ofj RCSB], [http://www.ebi.ac.uk/pdbsum/4ofj PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Staphylococcus aureus possesses two canonical ABC-importers dedicated to nickel acquisition: the NikABCDE and the CntABCDF systems, active under different growth conditions. This study reports on the extracytoplasmic nickel-binding components SaNikA and SaCntA. We showed by protein crystallography that SaNikA is able to bind either a Ni-(l-His)2 complex or a Ni-(l-His) (2-methyl-thiazolidine dicarboxylate) complex, depending on their availability in culture supernatants. Native mass spectrometry experiments on SaCntA revealed that it binds the Ni(ii) ion via a different histidine-dependent chelator but it cannot bind Ni-(l-His)2. In vitro experiments are consistent with in vivo nickel content measurements that showed that l-histidine has a high positive impact on nickel import via the Cnt system. These results suggest that although both systems may require free histidine, they use different strategies to import nickel. | ||
| - | + | Novel insights into nickel import in Staphylococcus aureus: the positive role of free histidine and structural characterization of a new thiazolidine-type nickel chelator.,Lebrette H, Borezee-Durant E, Martin L, Richaud P, Boeri Erba E, Cavazza C Metallomics. 2015 Jan 22. PMID:25611161<ref>PMID:25611161</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | + | <references/> | |
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Cavazza, C]] | [[Category: Cavazza, C]] | ||
[[Category: Lebrette, H]] | [[Category: Lebrette, H]] | ||
| + | [[Category: Abc-type importer]] | ||
| + | [[Category: Extracytoplasmic]] | ||
| + | [[Category: Extracytoplasmic nickel-binding protein]] | ||
| + | [[Category: Metal transport]] | ||
| + | [[Category: Nickel import]] | ||
| + | [[Category: Transport protein]] | ||
Revision as of 15:50, 7 February 2015
Crystal structure of NikA from Staphylococcus aureus in complex with Ni(L-His)2
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