2fxh
From Proteopedia
| Line 4: | Line 4: | ||
|PDB= 2fxh |SIZE=350|CAPTION= <scene name='initialview01'>2fxh</scene>, resolution 1.90Å | |PDB= 2fxh |SIZE=350|CAPTION= <scene name='initialview01'>2fxh</scene>, resolution 1.90Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Catalase Catalase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.6 1.11.1.6] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Catalase Catalase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.6 1.11.1.6] </span> |
|GENE= katG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=28450 Burkholderia pseudomallei]) | |GENE= katG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=28450 Burkholderia pseudomallei]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1mwv|1MWV]], [[2fxg|2FXG]], [[2fxj|2FXJ]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fxh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fxh OCA], [http://www.ebi.ac.uk/pdbsum/2fxh PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2fxh RCSB]</span> | ||
}} | }} | ||
| Line 31: | Line 34: | ||
[[Category: Singh, R.]] | [[Category: Singh, R.]] | ||
[[Category: Wiseman, B.]] | [[Category: Wiseman, B.]] | ||
| - | [[Category: HEM]] | ||
| - | [[Category: MPD]] | ||
| - | [[Category: NA]] | ||
| - | [[Category: TRS]] | ||
[[Category: catalase-peroxidase]] | [[Category: catalase-peroxidase]] | ||
[[Category: isoniazid]] | [[Category: isoniazid]] | ||
| Line 40: | Line 39: | ||
[[Category: tuberculosis]] | [[Category: tuberculosis]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:09:01 2008'' |
Revision as of 00:09, 31 March 2008
| |||||||
| , resolution 1.90Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , | ||||||
| Gene: | katG (Burkholderia pseudomallei) | ||||||
| Activity: | Catalase, with EC number 1.11.1.6 | ||||||
| Related: | 1MWV, 2FXG, 2FXJ
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of KatG at pH 6.5
Overview
Crystals of Burkholderia pseudomallei KatG retain their ability to diffract X-rays at high resolution after adjustment of the pH from 5.6 to 4.5, 6.5, 7.5, and 8.5, providing a unique view of the effect of pH on protein structure. One significant pH-sensitive change lies in the appearance of a perhydroxy group attached to the indole nitrogen of the active site Trp111 above pH 7, similar to a modification originally observed in the Ser324Thr variant of the enzyme at pH 5.6. The modification forms rapidly from molecular oxygen in the buffer with 100% occupancy after one minute of soaking of the crystal at room temperature and pH 8.5. The low temperature (4 K) ferric EPR spectra of the resting enzyme, being very sensitive to changes in the heme iron microenvironment, confirm the presence of the modification above pH 7 in native enzyme and variants lacking Arg426 or Met264 and its absence in variants lacking Trp111 or Tyr238. The indole-perhydroxy group is very likely the reactive intermediate of molecular oxygen in the NADH oxidase reaction, and Arg426 is required for its reduction. The second significant pH-sensitive change involves the buried side chain of Arg426 that changes from one predominant conformation at low pH to a second at high pH. The pH profiles of the peroxidase, catalase, and NADH oxidase reactions can be correlated with the distribution of Arg426 conformations. Other pH-induced structural changes include a number of surface-situated side chains, but there is only one change involving a displacement of main chain atoms triggered by the protonation of His53 in a deep pocket in the vicinity of the molecular 2-fold axis.
About this Structure
2FXH is a Single protein structure of sequence from Burkholderia pseudomallei. Full crystallographic information is available from OCA.
Reference
Roles for Arg426 and Trp111 in the modulation of NADH oxidase activity of the catalase-peroxidase KatG from Burkholderia pseudomallei inferred from pH-induced structural changes., Carpena X, Wiseman B, Deemagarn T, Herguedas B, Ivancich A, Singh R, Loewen PC, Fita I, Biochemistry. 2006 Apr 25;45(16):5171-9. PMID:16618106
Page seeded by OCA on Mon Mar 31 03:09:01 2008
