2g1g
From Proteopedia
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|PDB= 2g1g |SIZE=350|CAPTION= <scene name='initialview01'>2g1g</scene> | |PDB= 2g1g |SIZE=350|CAPTION= <scene name='initialview01'>2g1g</scene> | ||
|SITE= | |SITE= | ||
| - | |LIGAND= | + | |LIGAND= <scene name='pdbligand=A:ADENOSINE-5'-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5'-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5'-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=T6A:N-[N-(9-B-D-RIBOFURANOSYLPURIN-6-YL)CARBAMOYL]THREONINE-5'-MONOPHOSPHATE'>T6A</scene>, <scene name='pdbligand=U:URIDINE-5'-MONOPHOSPHATE'>U</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1feq|1FEQ]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2g1g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g1g OCA], [http://www.ebi.ac.uk/pdbsum/2g1g PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2g1g RCSB]</span> | ||
}} | }} | ||
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[[Category: t-rnai anticodonloop t6a]] | [[Category: t-rnai anticodonloop t6a]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:10:29 2008'' |
Revision as of 00:10, 31 March 2008
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| Ligands: | , , , , | ||||||
| Related: | 1FEQ
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Solution structure of the anticodon loop of S. Pombe tRNAi including the naturally occurring N6-threonyl adenine
Overview
Modified nucleosides play an important role in structure and function of tRNA. We have determined the solution structure of the anticodon stem-loop (ASL) of initiator tRNA of Schizosaccharomyces pombe. The incorporation of N6-threonylcarbamoyladenosine at the position 3' to the anticodon triplet (t6A37) results in the formation of a U-turn motif and enhances stacking interactions within the loop and stem regions (i.e. between A35 and t6A37) by bulging out U36. This conformation was not observed in a crystal structure of tRNAi including the same modification in its anticodon loop, nor in the solution structure of the unmodified ASL. A t6A modification also occurs in the well studied anti-stem-loop of lys-tRNA(UUU). A comparison of this stem-loop with our structure demonstrates different effects of the modification depending on the loop sequence.
About this Structure
2G1G is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
The naturally occurring N6-threonyl adenine in anticodon loop of Schizosaccharomyces pombe tRNAi causes formation of a unique U-turn motif., Lescrinier E, Nauwelaerts K, Zanier K, Poesen K, Sattler M, Herdewijn P, Nucleic Acids Res. 2006 May 31;34(10):2878-86. Print 2006. PMID:16738127
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