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2g23
From Proteopedia
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|PDB= 2g23 |SIZE=350|CAPTION= <scene name='initialview01'>2g23</scene>, resolution 2.30Å | |PDB= 2g23 |SIZE=350|CAPTION= <scene name='initialview01'>2g23</scene>, resolution 2.30Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=C2O:CU-O-CU+LINKAGE'>C2O</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= phsA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1890 Streptomyces antibioticus]) | |GENE= phsA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1890 Streptomyces antibioticus]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2g23 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g23 OCA], [http://www.ebi.ac.uk/pdbsum/2g23 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2g23 RCSB]</span> | ||
}} | }} | ||
| Line 27: | Line 30: | ||
[[Category: Smith, A W.]] | [[Category: Smith, A W.]] | ||
[[Category: Wang, M.]] | [[Category: Wang, M.]] | ||
| - | [[Category: C2O]] | ||
| - | [[Category: CU]] | ||
| - | [[Category: GOL]] | ||
[[Category: copper]] | [[Category: copper]] | ||
[[Category: hexamer]] | [[Category: hexamer]] | ||
| Line 36: | Line 36: | ||
[[Category: multicopper oxidase]] | [[Category: multicopper oxidase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:10:47 2008'' |
Revision as of 00:10, 31 March 2008
| |||||||
| , resolution 2.30Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , | ||||||
| Gene: | phsA (Streptomyces antibioticus) | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
The crystal structure of hexameric phenoxazinone synthase
Overview
The multicopper oxidase phenoxazinone synthase (PHS) catalyzes the penultimate step in the biosynthesis of the antibiotic actinomycin D by Streptomyces antibioticus. PHS exists in two oligomeric forms: a dimeric form and a hexameric form, with older actinomycin-producing cultures containing predominately the hexameric form. The structure of hexameric PHS has been determined using X-ray diffraction to a resolution limit of 2.30 A and is found to contain several unexpected and distinctive features. The structure forms a hexameric ring that is centered on a pseudo 6-fold axis and has an outer diameter of 185 A with a large central cavity that has a diameter of 50 A. This hexameric structure is stabilized by a long loop connecting two domains; bound to this long loop is a fifth copper atom that is present as a type 2 copper. This copper atom is not present in any other multicopper oxidase, and its presence appears to stabilize the hexameric structure.
About this Structure
2G23 is a Single protein structure of sequence from Streptomyces antibioticus. Full crystallographic information is available from OCA.
Reference
Structure of phenoxazinone synthase from Streptomyces antibioticus reveals a new type 2 copper center., Smith AW, Camara-Artigas A, Wang M, Allen JP, Francisco WA, Biochemistry. 2006 Apr 11;45(14):4378-87. PMID:16584173
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