3ntd

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(Difference between revisions)

Revision as of 02:01, 5 August 2016

Structure of the Shewanella loihica PV-4 NADH-dependent persulfide reductase C531S Mutant

Structural highlights

3ntd is a 2 chain structure with sequence from Shelp. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, ,
Related:	3nta, 3nt6
Gene:	Shew_0729 (SHELP)
Activity:	CoA-disulfide reductase, with EC number 1.8.1.14
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

The NADH-dependent persulfide reductase (Npsr), a recently discovered member of the PNDOR family of flavoproteins that contains a rhodanese domain in addition to the canonical flavoprotein reductase domain, is proposed to be involved in the dissimilatory reduction of S0 in Shewanella loihica PV-4, a facultative anaerobe capable of utilizing a wide range of respiratory substrates. We have previously shown that polysulfide is able to act as a substrate for this enzyme, and a recently determined structure of a closely related enzyme (CoADR-Rhod from Bacillus anthracis) suggested the importance of a bound coenzyme A in the mechanism. The work described here shows that small thiol persulfides, such as CoA and glutathione persulfides, are the likely in vivo oxidizing substrates for Npsr is and suggests that persulfides may play a central role in the respiration of S0. C43S, C531S and C43,531S mutants were created to determine the role of the flavoprotein domain cysteine (C43) and the rhodanese domain cysteine (C531) in the mechanism of the enzyme. The absolute requirement for C43 for persulfide or DTNB reductase activity indicates that this residue is involved in S-S bond breakage. C531 contributes to, but is not required for, catalysis of DTNB reduction, while it is absolutely required for reduction of any persulfide substrates. Titrations of the enzyme with NADH, dithionite, titanium(III) and TCEP demonstrate the presence of a mixed-disulfide between C43 and a tightly bound CoA, and structures of the C43 and C43,531S mutants confirm that this coenzyme A remains tightly bound to the enzyme in the absence of a C43-CoA S-S bond. The 2.0 A structures of wild-type and mutant Npsr suggests a likely site for persulfide substrate binding and reaction with the rhodanese domain cysteine. Based on kinetic, titration and structural data a mechanism for the reduction of persulfides by Npsr is proposed.

Characterization of an NADH-dependent persulfide reductase from Shewanella loihica PV-4: Implications for the mechanism of sulfur respiration via FAD-dependent enzymes.,Warner MD, Lukose V, Lee KH, Lopez K, Sazinsky M, Crane Iii EJ Biochemistry. 2010 Nov 23. PMID:21090815^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Warner MD, Lukose V, Lee KH, Lopez K, Sazinsky M, Crane Iii EJ. Characterization of an NADH-dependent persulfide reductase from Shewanella loihica PV-4: Implications for the mechanism of sulfur respiration via FAD-dependent enzymes. Biochemistry. 2010 Nov 23. PMID:21090815 doi:10.1021/bi101232y

1 Structural highlights
2 Publication Abstract from PubMed
3 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3ntd"

@@ Line 1: / Line 1: @@
 ==Structure of the Shewanella loihica PV-4 NADH-dependent persulfide reductase C531S Mutant==
 <StructureSection load='3ntd' size='340' side='right' caption='[[3ntd]], [[Resolution|resolution]] 1.99&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3ntd]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Shewanella_loihica Shewanella loihica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NTD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3NTD FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3ntd]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Shelp Shelp]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NTD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3NTD FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3nta|3nta]], [[3nt6|3nt6]]</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Shew_0729 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=359303 Shewanella loihica])</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Shew_0729 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=323850 SHELP])</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/CoA-disulfide_reductase CoA-disulfide reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.8.1.14 1.8.1.14] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ntd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ntd OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ntd RCSB], [http://www.ebi.ac.uk/pdbsum/3ntd PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ntd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ntd OCA], [http://pdbe.org/3ntd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ntd RCSB], [http://www.ebi.ac.uk/pdbsum/3ntd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ntd ProSAT]</span></td></tr>
 </table>
 <div style="background-color:#fffaf0;">
@@ Line 17: / Line 18: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 3ntd" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>
@@ Line 22: / Line 24: @@
 </StructureSection>
 [[Category: CoA-disulfide reductase]]
-[[Category: Shewanella loihica]]
+[[Category: Shelp]]
 [[Category: Crane, E J]]
 [[Category: Lee, K H]]

3ntd

From Proteopedia

Revision as of 02:01, 5 August 2016

Structure of the Shewanella loihica PV-4 NADH-dependent persulfide reductase C531S Mutant

Structural highlights

Publication Abstract from PubMed

References

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Proteopedia Page Contributors and Editors (what is this?)

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